The Effects of Leptin on Proliferation and Function of Human Osteoblast
10.3969/j.issn.1672-173X.2001.02.024
- VernacularTitle:Leptin对人成骨细胞增殖和功能的影响
- Author:
Shuangqing LI
1
;
Hongli ZHAO
;
Songquan WEI
;
Zhenmei AN
;
Qin XIE
;
Xiaojia LI
;
Weiguo JIA
Author Information
1. The Gold Card Hospital The First Affiliated Hospital WCUMS
- From:
Journal of Sichuan University (Medical Sciences)
2001;32(2):240-242
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of leptin on osteoblast. Methods Human osteoblast primary culture was carried out, and the morphology and function of osteoblast were observed. The effects of different levels of leptin on osteoblast in different days were assessed by MTT colorimetry. Osteocalcin production was measured also. Results Human osteoblasts were fusiform in shape and were positive for alkaline phosphatase by histochemical staining, positive for osteocalcin by immunofluorescence staining, and positive by Alizarin Reds staining after mineralized upon supplementation with ascorbate and β-glycerophosphate. On the first, second and third days, the prolifertion of osteoblast， cultured with different concentrations of leptin， had no changes. The leptin-stimulated synthesis of osteocalcin of cells was found to be dose-dependent (P＜0.05), but not time-dependent (P＞0.05). Conclusion The above data indicated that there were no evidences for the effects of leptin on the proliferation of human osteoblast, but leptin could enhance the function of human osteoblast.
