Study on Donor-Specific Antigens in Inducing Immunogical Tolerance
10.3969/j.issn.1672-173X.2001.02.019
- VernacularTitle:供者特异性抗原诱导免疫耐受的实验研究
- Author:
Ming SHI
1
;
Jia WANG
;
Xiang LI
;
Li WANG
;
Hong SHEN
;
Yiping LU
;
Yuru YANG
Author Information
1. the First Affiliated Hospital WCUMS
- From:
Journal of Sichuan University (Medical Sciences)
2001;32(2):222-224
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the effects of different donor-specific antigens, through different pathways, on the inducement of immunological tolerance. Methods After the establishment of a stable rat cervical heterotopic heart transplantation model, donor specific transfusion (DST), donor specific spolenocyte (DSSL) and donor specific bone marrow (DSBM) were given to recipients 3-14 days prior to the transplantation through peripheral vein, portal vein and intrathymic injection respectiviely. Low dose CsA 5mg/(kg*d) was given from the 3rd-14th day pre-transplantation to the 28th day post-transplantation. The survival time of cardiac grafts was monitored, meanwhile, the donor specific mixed lymphocyte reaction (MLR) and the subsets of T lymphocytes CD8+ were determined. Results DSBM and the administrative pathway of intrathymic injection achieved the best effects on the inducement of immunological tolerance and the improvement of graft survival. Conclusion The donor specific antigens can induce the donor-specific immunological tolerance. It may be a practicable approach for the clinical experiments in the future.
