Construction of Recombinant Plasmids Containing Genes of HBsAg and Their Expression in the Eukaryotic Cells
10.3969/j.issn.1672-173X.2001.02.004
- VernacularTitle:乙肝基因疫苗系列质粒的构建及其在真核细胞中的表达
- Author:
Shan QIN
1
;
Hong TANG
;
Liansan ZHAO
;
Li LIU
;
Yong LIN
Author Information
1. The First Affiliated Hospital WCUMS
- From:
Journal of Sichuan University (Medical Sciences)
2001;32(2):172-174
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct recombinant plasmids expressing L,M,S and pre-S1-S protein of HBsAg. Methods Amplifying segments of S, pre-S2-S, pre-S1-pre-S2-S genes of HBV by PCR and amplifying segment of pre-S1-S by overlap extension PCR; inserting the segments into Rc/CMV and pSG5UTPL/Flag plasmids respectively and exploring their expressions by Western-Blot hybridization，identifying the inserting segments by sequencing.Results The sequences of the inserted segments were the same as the genes of S, pre-S2-S, pre-S1-pre-S2-S and pre-S1-S and the results of Western-Blot hybridization were positive for the aimed proteins. Conclusion We have gained 8 recombinant plasmids expressing S, M, L and pre-S1-S proteins with high efficacy.
