Investigating the effects and mechanisms of Yiqi Jiedu Decoction in protecting against ionizing radiation—induced small intestinal functional damage in mice based on ferroptosis
10.16289/j.cnki.1002-0837.2025.05001
- VernacularTitle:基于铁死亡探究益气解毒方防护电离辐射致小鼠小肠功能损伤的效应及机制
- Author:
Yan WANG
1
;
Minhao XU
;
Wenyan ZHANG
;
Yuankai GAO
;
Qing XU
;
An WANG
;
Wenhui XU
;
Sumin HU
Author Information
1. 北京中医药大学中医学院,北京 102488
- Keywords:
ionizing radiation;
ferroptosis;
small intestine;
Yiqi Jiedu Decoction
- From:Space Medicine & Medical Engineering
2025;36(5):389-395
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the protective effects of Yiqi Jiedu Decoction on ionizing radiation-induced small intestinal functional injury in mice,and explore whether it alleviates such injury by inhibiting small intestinal ferroptosis,thereby providing scientific support for the discovery and development of intestinal radiation protection drugs in aerospace medicine.Methods A total of 378 male Balb/c mice were randomly divided into 7 groups:blank control group,model group,positive drug group,high-dose Yiqi Jiedu Decoction group,low-dose Yiqi Jiedu Decoction group,Liproxstatin-1 pre-irradiation administration group,and Liproxstatin-1 post-irradiation administration group,with 54 mice in each group.Each group was further divided into 3 batches,with 18 mice per batch.Seven days after preventive administration,all groups except the blank control group were subjected to a single whole-body irradiation with 2.0 Gy 60Co γ-rays.The general condition and morphological structure of the small intestine were observed at 1,3,and 7 days post-irradiation.The small intestinal charcoal propulsion rate,serum D-xylose content,and lactic acid content were measured,along with the levels of Fe,LPO,MDA,GSH,and SOD activity in the small intestine.Results Yiqi Jiedu Decoction could mitigate the decrease in body weight of mice after 2.0 Gy 60Co γ-ray irradiation,improve the morphological structure of the small intestine,reduce the small intestine charcoal propulsion rate,increase serum D-xylose levels,and decrease total serum lactate levels.It also alleviated mitochondrial shrinkage in the small intestine and reduced the contents of Fe and MDA in small intestine tissues.Conclusion Yiqi Jiedu Decoction may alleviate ionizing radiation-induced small intestinal functional injury by inhibiting ferroptosis in the small intestine,providing a new strategy for intestinal radiation injury in deep space exploration missions such as manned spaceflight.
