Regulatory effect and mechanism of fuping on macrophage M1/M2 polarization in preventing and treating ionizing radiation-induced inflammatory damage to the immune system in mice
10.16289/j.cnki.1002-0837.2025.04016
- VernacularTitle:浮萍调控巨噬细胞M1/M2极化防治电离辐射致小鼠炎症损伤的效应及机制研究
- Author:
Zhangdi ZHAO
1
;
An WANG
;
Xiaoying CHEN
;
Shuran WANG
;
Sumin HU
Author Information
1. 北京中医药大学中医学院,北京 100029
- Keywords:
fuping;
macrophage;
ionizing radiation;
polarization;
inflammatory injury
- From:Space Medicine & Medical Engineering
2025;36(4):372-379
- CountryChina
- Language:Chinese
-
Abstract:
Objective With the continuous advancement of China's deep space exploration and space station construction missions,the issues of radiation protection and health security for astronauts in special space environments have become increasingly prominent,gradually emerging as a core research topic in the field of special medicine.To investigate the protective effect and mechanism of Fuping decoction against ionizing radiation-induced inflammatory damage to the immune system in mice,and to verify whether it relieves radiation-induced inflammatory injury by regulating macrophage M1/M2 polarization balance.Methods An in vivo radiation injury model was established using Balb/c mice.The expression levels of pro-inflammatory factors TNF-α and IL-6 in the spleen and thymus,and the phagocytic rate of peritoneal macrophages were detected.Mouse-derived macrophages were cultured in vitro to establish an in vitro radiation injury model.The secretion levels of inflammatory factors TNF-α,IL-6,and IL-10 were measured,macrophage polarization was observed by immunofluorescence,and the protein expression of the TLR4/MyD88/NF-κB signaling pathway was analyzed by Western blot.Results In vivo experiments showed that Fuping significantly reduced the phagocytic rate of peritoneal macrophages and the contents of TNF-α and IL-6 in the spleen and thymus of irradiated mice.In vitro experiments demonstrated that Fuping restored the M1/M2 polarization balance of macrophages,decreased the expression levels of TNF-α and IL-6,increased the expression of IL-10,and inhibited the protein expression of TLR4,MyD88,and NF-κB in irradiated macrophages.Conclusion Fuping alleviates radiation-induced inflammatory injury by inhibiting the TLR4/MyD88/NF-κB signaling pathway and regulating macrophage polarization balance,providing an experimental basis for the development of natural radioprotective drugs.
