Research on the changes of energy metabolism substrates under fasting hypometabolic state in rats
10.16289/j.cnki.1002-0837.2025.01006
- VernacularTitle:禁食低代谢状态下大鼠能量代谢底物变化规律的研究
- Author:
Xiukun SUI
1
;
Feng WU
;
Siyu JIANG
;
Hailong WANG
;
Hongyu ZHANG
;
Chao YANG
;
Yaxiu GUO
;
Yinghui LI
;
Zhongquan DAI
Author Information
1. 中国航天员科研训练中心航天医学全国重点实验室,北京 100094
- Keywords:
fasting hypometabolism;
biochemical testing;
glucose and lipid metabolism;
energy metabolism homeostasis
- From:Space Medicine & Medical Engineering
2025;36(1):32-37,42
- CountryChina
- Language:Chinese
-
Abstract:
Objective Fasting hypometabolism regulation technology has broad application potential in long-term space flight and survival in extreme extraterrestrial environments.In-depth research on the substrate conversion of energy metabolism and the formation of new steady states under fasting hypometabolism will provide theoretical basis and experimental data support for formulating effective prolonged fasting application mode.Methods 30 SD rats were randomly divided into control group and fasting group(fasting for 1,2,3,and 5 days).Blood biochemical examination,qRT-PCR,and western blotting were performed to analyze the body weight,blood biochemistry,and expression changes of genes and proteins related to glucose and lipid metabolism during different fasting periods.Results Prolonged fasting significantly reduced the body weight,blood glucose,and triglyceride levels of rats;increased the blood ketone level,and replaced glucose as the main energy substance in the body.There are temporal and tissue-specific changes as a whole.Hepatic and renal gluconeogenesis play major roles respectively during different fasting periods.As the fasting time prolongs,the level of hepatic gluconeogenesis gradually decreases,the content of FFA in the blood increases,the expression level of genes related to fat synthesis decreases,fatty acid oxidation is enhanced,and the expression level of the key gene HMGCS2 for ketone body generation increases.Conclusion During prolonged fasting,there is a significant conversion of glucose-ketone energy supply substrates,and a new steady state of energy metabolism mainly supplied by ketone bodies is formed within 2-5 days of fasting.The body maintains a low metabolic state by regulating changes in key genes in pathways such as glucose and lipid metabolism.
