Poly(dimethyl siloxane)surface modification based on oxygen plasma treatment and physical adsorption
10.16289/j.cnki.1002-0837.2024.06004
- VernacularTitle:氧等离子体处理与物理吸附相结合的聚二甲基硅氧烷表面改性
- Author:
Liujia SHI
1
;
Yingjun TAN
;
Chunyan WANG
;
Jielin NIE
;
Yin GU
;
Taowan GONG
;
Zi XU
;
Chengjia YANG
;
Bo WANG
;
Bai DING
;
Lina QU
;
Yinghui LI
Author Information
1. 中国航天员科研训练中心,北京 100094
- Keywords:
poly(dimethyl siloxane);
surface modification;
oxygen plasma treatment;
physical adsorption
- From:Space Medicine & Medical Engineering
2024;35(6):352-356
- CountryChina
- Language:Chinese
-
Abstract:
Poly(dimethyl siloxane)(PDMS)is suitable for the fabrication of space cell culture chips.It is necessary to perform highly stable hydrophilic modification to promote cell adhesion on PDMS surface.We modified PDMS surface by oxygen plasma treatment combining with physical adsorption in this article.PDMS,which was treated with oxygen plasma and sequentially coated with Poly-L-lysine or collagen(PLL-ox-PDMS,COL-ox-PDMS)with various solution concentration and kept in different environmental conditions,were characterized by contact angle and surface energy measurements,and atomic force microscopy(AFM).Cell growth conditions on bare PDMS and modified PDMS were compared,and the numbers of live cells cultured for 1-3 days were evaluated.Experimental results indicate that higher solution concentration and low storage temperature benefit the hydrophilicity stability.The contact angles of PLL-ox-PDMS stored in 4℃and COL-ox-PDMS were close to 60°and 65°on day 25 and 14,respectively.Both modified PDMS surfaces are suitable for cell adhesion and proliferation,and no cell layer falls offin 10 days.The modification method is especially suitable for applications such as medium and long term space cell culture.
