The effect of microgravity on hibernating myoblasts
- VernacularTitle:微重力对休眠成肌细胞的影响
- Author:
Yizhou LIU
1
;
Xiaojian CAO
;
Liujia SHI
;
Yunqiang CHEN
;
Yingjun TAN
;
Danxia HUANG
;
Chunyan WANG
;
Qiuzhi ZHOU
;
Lina QU
;
Hongmei LUO
;
Xuemin YIN
;
Song ZHANG
;
Zhaoxia LIU
;
Yajie LI
;
Jia XU
;
Yinghui LI
;
Hong CHEN
Author Information
- Keywords: hibernation; myoblasts; microgravity; space flight; skeleton muscle regeneration
- From:Space Medicine & Medical Engineering 2024;35(5):275-281
- CountryChina
- Language:Chinese
- Abstract: Objective To investigate the effects of microgravity environment on hibernating myoblasts.Methods Hibernating myoblasts were cultured under real and simulated microgravity conditions for 10 days.RNA-seq analysis and immunofluorescence are used to analysis the impact of microgravity environment on cell growth and gene expression of myoblasts.Results Under the microgravity conditions,genes associated with proliferation were upregulated.Under simulated microgravity,there were more and higher proportion of Ki67 positive cells compared to normal gravity conditions.Conclusion The microgravity environment promotes the proliferation of hibernating myoblasts.
