Application value of blood miR-192,PD-1,Tim-3,and Wnt signaling pathway-related factors in clinic staging of syphilis
10.3969/j.issn.1009-0754.2025.08.012
- VernacularTitle:血miR-192、PD-1、Tim-3及Wnt信号通路相关因子表达情况在梅毒分期中的应用价值评估
- Author:
Li DONG
1
;
Yuanyuan DONG
;
Miao HE
Author Information
1. 214000 江苏无锡,无锡市第五人民医院检验科
- Keywords:
Syphilis staging;
Tim-3;
miR-192;
PD-1;
Wnt signaling pathway;
Prediction
- From:
Journal of Navy Medicine
2025;46(8):805-810
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the application value of the expression of microRNA-192(miR-192),programmed cell death protein 1(PD-1),T-cell immunoglobulin and mucin-domain containing-3(Tim-3),Wnt family member 1(Wnt1),β-catenin,and Dickkopf-related protein 1(DKK1)in the clinic staging of syphilis,so as to provide more accurate biological markers for the diagnosis and staging of syphilis.Methods A total of 106 patients who were diagnosed as syphilis in the Fifth People's Hospital of Wuxi between September 2022 and September 2023 were assigned to the study group,and 48 healthy individuals who underwent physical examination in the same period were enrolled as controls.There were 68 males and 38 females with a mean age of(34.71±3.59)years in the study group.There were 26 males and 22 females with a mean age of(34.25±3.62)years in the control group.Quantitative PCR was used to detect to expression level of miR-192 in the blood of patients,and enzyme-linked immunosorbent assay(ELISA)was used to detect the expression of blood miR-192,PD-1,Tim-3,Wnt1,β-catenin,DKK1.These indicators were compared among patients with different stages of syphilis.The correlation of clinic staging of syphilis with miR-192,PD-1,Tim-3,and Wnt1 was investigated.Its predictive efficiency for syphilis staging was evaluated by receiver operator characteristic(ROC)curve.Results The expression levels of miR-192,Wnt1,and DKK1 in the study group were lower than those in the control group,while the levels of PD-1,Tim-3,and β-catenin were higher than those in the control group(P<0.05).The expression levels of miR-192,Wnt1,and DKK1 in the syphilis patients at different stages were as follows:latent stage>primary stage>secondary stage>tertiary stage;the expression levels of PD-1,Tim-3,and β-catenin in the syphilis patients at different stages were as follows:latent stage
