Effect of standing time on recovery rate of buffy coat pooled platelet
10.3969/j.issn.1009-0754.2025.06.018
- VernacularTitle:不同静置时间对汇集白膜层法制备冰冻血小板回收率的影响
- Author:
Muhan WANG
1
;
Zhanshan ZHA
;
Wenlong LI
;
Baohua QIAN
Author Information
1. 200433 上海,海军军医大学第一附属医院输血科
- Keywords:
Frozen platelet;
Pooled buffy coats;
Platelet recovery rate;
Residual red blood cells
- From:
Journal of Navy Medicine
2025;46(6):620-623
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the recovery rate of frozen platelets prepared by pooled buffy coats(PBCs)under different standing time points,so as to improve the preparation method of platelets.Methods The whole blood(400 ml)were collected from 50 blood donators,and was equally divided into 1-hour group(standing time of buffy coat pooled platelet for 1 h,n=50)and 24-hour group(standing time of buffy coat pooled platelet for 24 h,n=50).The concentrated platelets were stored at-80℃.The recovery rate and morphology of the platelet were compared between the two groups one month later.Results The platelet count and recovery rate of the frozen platelet in the 24-hour group were higher than those in the 1-hour group([2.45±0.13]×109 vs.[2.32±0.10]×109,83.55%±5.42%vs.79.32%±5.75%,both P<0.05).There was no significant difference in the average platelet volume,platelet distribution width,pH,P-selectin,or residual red blood cells between the two groups.Conclusion Residual red blood cells and platelet count from PBCs under different standing time points meet the national quality standards.The buffy coat pooled platelet count and recovery rate of 24-hour standing are higher than those of 1-hour standing.
