Metabolomic study of ginsenoside Rh1 against exercise-induced fatigue
10.3969/j.issn.1009-0754.2025.01.011
- VernacularTitle:人参皂苷Rh1抗运动性疲劳的代谢组学研究
- Author:
Jiaxuan LI
1
;
Xuefang WANG
;
Yinglu FENG
Author Information
1. 266007 山东青岛,青岛大学医学部中西医结合中心
- Keywords:
Ginsenoside Rh1;
Exercise-induced fatigue;
Metabolomics;
Tricarboxylic acid cycle
- From:
Journal of Navy Medicine
2025;46(1):46-51
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of ginsenoside Rh1 in regulating the changes of characteristic differential metabolites in rat models of exercise-induced fatigue by gas chromatography-mass spectrometry(GC-MS)and multidimensional statistical analysis,and to clarify the metabolic pathways,so as to provide experimental basis and theoretical support for the effective alleviation of exercise-induced fatigue through Rh1.Methods A total of 27 SPF SD rats were randomly divided into blank control group,model group,and ginsenoside Rh1 group,with 9 rats in each group.A rat exercise-induced fatigue model was established by treadmill exercise.After blood sampling,GC-MS technology,Principal Component Analysis(PCA)and Orthogonal Partial Least Squares(PLS-DA)were used for the identification and screening of differential metabolites,which were further structurally identified in KEGG and HMDB databases.MetPA database was used to construct metabolic pathways and enrich for topological analysis.Results The time to exhaustion in the ginsenoside Rh1 group was significantly longer than that in the model group.Significant differential expression of 8 metabolites(6 significantly decreased and 2 significantly increased)was found in the model group as compared to the blank control group,and 5 metabolic pathways were involved.In the ginsenoside Rh1 group,there were 3 metabolites with significant changes compared with the model group.Citric acid and fatty acid were significantly increased,α-D-glucosamine 1-phosphate was decreased,and the main pathway involved was tricarboxylic acid cycle.Conclusion Rh1 can significantly prolong the time to exhaustion and relieve exercise-induced fatigue in rats,which is closely related to the metabolic pathway of tricarboxylic acid cycle.
