Establishment of animal model of smoke inhalation injury combined with decompression sickness
10.3969/j.issn.1009-0754.2024.12.009
- VernacularTitle:烟雾吸入性损伤复合减压病的动物模型建立
- Author:
Guosheng WU
1
;
Honghao LAI
;
Ruiyong CHEN
;
Guangsheng LIU
;
Min LIU
;
Ying HE
Author Information
1. 200433 上海,海军军医大学第一附属医院烧伤外科
- Keywords:
Combined injury;
Inhalation injury;
Decompression sickness;
Animal model
- From:
Journal of Navy Medicine
2024;45(12):1261-1265
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore animal model and evaluation indices of inhalation injury combined with decompression sickness(DSC),so as to lay a foundation for the studies on damage effect,mechanism,prevention,and military medical service of this disease.Methods A total of 16 SD rats with similar physiological states were selected and randomly divided into four groups,including control group,DCS group,smoke inhalation injury(SII)group,and combined injury group(DCS after SII induced by a self-made device).Arterial blood gas,wet-dry ratio of lung tissue(W/D),and inflammatory cytokines in bronchoalveolar lavage fluid(BALF)like interleukin(IL)-1β IL-6,and tumor necrosis factor-α(TNF-α)were measured at 24 hours after modeling.And pulmonary histopathological staining was performed.Results The partial pressure of oxygcn in the combined injury group was lower than those in the other groups,while the partial pressure of arterial oxygen in the combined injury group was higher than those in the other groups(P<0.05).The results of pulmonary histopathology showed the thickening of the interlobular septa and the leakage and massive accumulation of inflammatory cells.The symptoms of acute lung injury in the combined injury group were more severe than those in the DCS and SII groups.The W/D and inflammatory cytokines in the combined injury group were significantly higher than those in the DCS group and SII group(P<0.05).Conclusion The animal model of SII combined with DCS has been successfully established.Pulmonary injury in the combined injury model is more severe than that in the single injury models.
