Mechanism of Sihuang powder in the treatment of knee synovitis by network pharmacology
10.3969/j.issn.1009-0754.2024.11.016
- VernacularTitle:基于网络药理学探讨四黄散治疗膝关节滑膜炎的作用机制
- Author:
Qiujia LIU
1
;
Weihong LI
;
Xiaolei LIU
;
Yue LIU
;
Xiaodong TANG
Author Information
1. 200433 上海,海军军医大学中医系
- Keywords:
Sihuang powder;
Knee synovitis;
Network pharmacology;
Molecular docking
- From:
Journal of Navy Medicine
2024;45(11):1178-1185
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of Sihuang powder(Radix et Rhizoma Rhei,Cortex Phellodendri,Radix Scutellariae and Fructus Gardeniae)in the treatment of knee synovitis by network pharmacology.Methods The active ingredients and corresponding targets of Sihuang powder were obtained from TCMSP database.A network diagram including medicines,active ingredients and targets was drawn by Cytoscape 3.9.1 software.The core targets were obtained by intersecting with the disease targets obtained from OMIM and Gene Cars databases.A target interaction network was constructed based on STRING database,and the GO/KEGG(gene ontology/Kyoto encyclopedia of genes and genomes)visualization analysis was performed on the core targets through DAVID database.The main chemical components and core target information were queried in TCMSP database and PDB database,and the results were imported into Autodock Tools.Autodock Vina was used for molecular docking analysis,and PyMol was used to draw 3D maps.Results A total of 68 active ingredients and 244 drug targets of Sihuang powder were obtained,including 18 core targets,such as tumor necrosis factor(TNF),interleukin-6(IL-6),protein kinase B1(AKT1)and tumor protein p53(TP53).A total of 118 signal pathways were obtained by KEGG enrichment analysis.The molecular docking results showed that quercetin,kaempferol,baicalein and wogonin in Sihuang powder had strong affinity with key targets.Conclusion Sihuang powder has the characteristics of multi-component,multi-target,and multi-pathway in the treatment of knee synovitis.It lays a foundation for further basic experimental research,and provides a reference for the exploration of the mechanism of this prescription in the treatment of knee synovitis.
