Differential expression of Acyl-CoA:cholesterol acyltransferase 1(ACAT1)in osteoarthritis and its genetic correlation analysis

VernacularTitle:酰基辅酶A:胆固醇酰基转移酶1在骨关节炎中的表达差异及其遗传相关性分析
Author: Wei HUANG ¹ ; Wei LU ; Xiaopeng XIA ; Nan LI ; Ying CHEN
Author Information

1. 226001 江苏南通,南通市中医院
Keywords: Osteoarthritis; Cholesterol; ACAT; Genetic correlation analysis
From: Journal of Navy Medicine 2024;45(6):612-617
CountryChina
Language:Chinese
Abstract: Objective To investigate the differential expression of Acyl-CoA:cholesterol acyltransferase 1(ACAT1)in osteoarthritis(OA)and its genetic correlation.Methods The expression of ACAT1 in synovial tissues of OA patients and OA mice was analyzed by using GEO database.The OA model was verified by haematoxylin-eosin(H-E)staining.The expression of ACAT1 was detected by RT-PCR.The highly synergistic genes of ACAT1 were analyzed by genome-wide genetic correlation analysis.Results The expression of ACAT1 was down-regulated in synovial tissues of OA patients and OA mice.The changes of ACAT1 expression in cartilage tissues of different strains of BXD and BXH RI mice showed a linear trend,indicating that the expression of ACAT1 gene presented with a typical quantitative genetic trait and was regulated by multiple factors.About 500 genes were highly correlated with ACAT1(P<0.05),including genes related to cell membrane structure,cell-to-cell connectivity and signaling pathways,gene transcription regulation,and protein stability.Conclusion ACAT1 may be a new target for the treatment of OA.