Determination of Baicalin in Chaiguijiebiao granule by high performance liquid chromatography
10.3969/j.issn.1009-0754.2024.01.018
- VernacularTitle:高效液相色谱法测定柴桂解表颗粒中黄芩苷含量的研究
- Author:
Cheng CHENG
1
;
Xiangmei CHEN
;
Jian MA
;
Yi ZHANG
Author Information
1. 100048 北京,解放军总医院医疗保障中心药剂科
- Keywords:
Chaiguijiebiao granule;
Baicalin;
High performance liquid chromatography;
Determination
- From:
Journal of Navy Medicine
2024;45(1):91-94
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for the determination of baicalin in Chaiguijiebiao granule by high performance liquid chromatography(HPLC)and further improve the quality standards of Chaiguijiebiao granule.Methods The sample of Chaiguijiebiao granule was treated by ultrasound to prepare the test product solution.Specificity test,linear relationship test,precision test,repeatability test,and sample recovery test were investigated by HPLC.On the basis of qualified methodology,the content of baicalin in 3 batches of Chaiguijiebiao granule was determined.Results The concentration of baicalin in the 3 batches of samples was 4.07 mg/g,4.11 mg/g and 4.40 mg/g,respectively.Preliminary quality standard of Chaiguijiebiao granule was established,with baicalin content ranging from 4.0 to 4.5 mg/g.Conclusion The HPLC method is simple,accurate and reliable,and can be used for the determination of baicalin in Chaiguijiebiao granule.
