Roles of telomerase,interferon-γ,adenosine deaminase and T cell spot test in diagnosis of tuberculous and malignant pleural effusion
10.3969/j.issn.1009-0754.2024.01.011
- VernacularTitle:端粒酶、γ-干扰素、腺苷酸脱氨酶及结核感染T细胞斑点试验在诊断结核性和恶性胸腔积液中的作用分析
- Author:
Zhenhua YOU
1
;
Jinhong HUANG
;
Yungen ZHAO
;
Yuping GU
;
Yi CHENG
;
Chunhua LING
Author Information
1. 215500 江苏常熟,常熟市第二人民医院呼吸内科
- Keywords:
Telomerase;
Interferon-γ;
Adenosine deaminase;
T cell spot test;
Tuberculous pleural effusion;
Malignant pleural effusion
- From:
Journal of Navy Medicine
2024;45(1):53-57
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the roles of telomerase,interferon-γ(IFN-γ),adenosine deaminase(ADA)and T cell spot test(T-SPOT.TB)in the diagnosis of tuberculous and malignant pleural effusion.Methods Forty patients with early tuberculous pleurisy(tuberculosis group)and 40 patients with malignant pleural effusion(tumor group)were enrolled.Pleural effusion and fasting blood were extracted before treatment.The activity of telomerase and the concentration of IFN-γ were detected by enzyme-linked immunosorbent assay.The activity of ADA was detected by colorimetric analysis.The positive rate of T-SPOT.TB was detected by Elispot spot counting.Results Telomerase activity in the tuberculosis group was significantly lower than that in the tumor group([1.46±0.73]×10-3 nmol/L vs.[3.34±1.72]×10-3 nmol/L,P<0.01).The concentration of IFN-γ was(137.44±38.93)U/L and(27.94±8.46)U/L in the tuberculosis group and tumor group,respectively;ADA content was(68.42±9.58)U/L and(15.39±4.43)U/L.There were significant differences in IFN-γ and ADA between the 2 group(P<0.01).IFN-γ concentration,ADA activity and the positive rate of T-SPOT.TB in the tuberculosis group were significantly higher than those in the tumor group(P<0.01).The sensitivity and specificity of combined detection of telomerase,IFN-γ,ADA and T-SPOT.TB in differentiating tuberculous and malignant pleural effusion were 0.974 and 0.974,respectively.Conclusion The combined detection of telomerase,IFN-γ,ADA and T-SPOT.TB has important value in differential diagnosis of tuberculosis and malignant pleural effusion.
