Relationship between the expression of NF-κB family P50 and IκB-α in cervical lesions and HPV16-positive cervical cancer
10.3969/j.issn.1009-0754.2023.11.015
- VernacularTitle:宫颈病变中转录因子NF-κB家族P50、IκB-α表达与HPV16阳性宫颈癌的关系分析
- Author:
Lingling LI
1
;
Yi YIN
;
Aiqin HE
Author Information
1. 226000 江苏 南通,南通大学附属肿瘤医院肿瘤妇科
- Keywords:
Human papilloma virus;
Cervical cancer;
Transcription factor;
Nuclear factor κB;
NF-κB inhibitory protein-α;
Prognosis
- From:
Journal of Navy Medicine
2023;44(11):1173-1178
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the relationship between the expression of nuclear factor κB(NF-κB)family of transcription factors(P50)and NF-κB inhibitory protein-α(IκB-α)in cervical lesions and human papilloma virus 16(HPV16)-positive cervical cancer.Methods Cervical tissue samples were collected from 106 patients with HPV16-positive cervical cancer,52 patients with high-grade squamous intraepithelial neoplasia(HSIL),and 43 patients with low-grade squamous intraepithelial neoplasia(LSIL)admitted to Tumor Hospital Affilated to Natong University from March 2017 to February 2019.Normal cervical tissue samples were collected from 80 patients who underwent total hysterectomy for adenomyosis during the same period.The expression levels of NF-κB P50 and IκB-α in different cervical tissue samples were determined by immunohistochemistry.The expression of NF-κB P50 and IκB-α in different cervical tissue was compared.The relationship between NF-κB P50 and IκB-α expression levels and pathological features of cervical cancer patients was investigated.Cox regression analysis was used to assess the factors affecting the prognosis of patients with HPV16-positive cervical cancer.The relationship between NF-κB P50 and IκB-α expression levels and the prognosis of HPV16-positive cervical cancer patients was analyzed.Results The positive expression rate of NF-κB P50 and the negative expression rate of IκB-α from high to low was:cervical cancer tissue,HSIL tissue,LSIL tissue,and normal cervical tissue(P<0.01).The positive expression rate of NF-κB P50 and the negative expression rate of IκB-α in HPV16-positive cervical cancer patients with the largest tumor diameter≥4 cm,clinical stage III/IV,lymph node metastasis,poorly differentiated,and vascular invasion were significantly higher than those in the patients with the tumor largest diameter<4 cm,clinical stage I/II,no lymph node metastasis,moderate/well differentiated,and no vascular invasion(P<0.05).Cox regression analysis showed that clinical stage(OR:2.546,95%CI:1.054-3.672),lymph node metastasis(OR:2.734,95%CI:1.469-4.183),degree of differentiation(OR:3.257,95%CI:2.015-6.432),positive expression of NF-κB P50(OR:4.025,95%CI:2.765-8.436),and negative expression of IκB-α(OR:4.368,95%CI:3.104-10.254)were independent influence factors for the prognosis of patients with HPV16-positive cervical cancer factor(P<0.05).By the end of the follow-up,the overall survival rates of NF-κB P50 positive patients and NF-κB P50 negative patients were 73.68%and 92.59%,respectively.There was a statistically significant difference between the survival curves of patients with positive NF-κB P50 expression and those with negative NF-κB P50 expression(P<0.05).The overall survival rates of IκB-α positive patients and IκB-α negative patients were 100.00%and 76.34%,respectively.There was a statistically significant difference between the survival curves of patients with positive IκB-α protein expression and those with negative expression of IκB-α protein(P<0.05).Conclusion There are high positive expression rate of NF-κB P50 and high negative expression rate of IκB-α in the cancer tissue of HPV16-positive cervical cancer patients.The expression of NF-κB P50 and IκB-α has a certain correlation with clinicopathological characteristics and prognosis of HPV16-positive cervical cancer patients.
