Analysis of intestinal flora imbalance as an initial factor in the occurrence and development of diarrhea-predominant irritable bowel syndrome
10.3969/j.issn.1009-0754.2023.11.014
- VernacularTitle:肠道菌群失调作为始发因素参与腹泻型肠易激综合征的发生与发展分析
- Author:
Changhao DONG
1
;
Chao LI
;
Shaoxin WANG
;
Guangxiang WANG
;
Rui XIAN
;
Xiaona LIU
;
Lihong CUI
Author Information
1. 510006 广东广州,华南理工大学医学院
- Keywords:
Intestinal flora;
Irritable bowel syndrome with diarrhea;
Inflammation
- From:
Journal of Navy Medicine
2023;44(11):1168-1172
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of intestinal flora in the development of irritable bowel syndrome with diarrhea(IBS-D).Methods The mice treated with antibiotics were randomly assigned to experimental group or control group.IBS-D mouse feces and normal mouse feces were given to the experimental group and control group for fecal microbiota transplantation,respectively.The fecal water content,intestinal transit time,and intestinal sensitivity were observed.qRT-PCR was used to determine the mRNA expression of nuclear factor kappa-B(NF-κB),Toll like receptor 4(TLR4),myeloid differentiation factor 88(MyD88),interleukin-1β(IL-Iβ),interleukin-6(IL-6),and tumor necrosis factor α(TNF-α)in colon tissue.Western blotting was used to determine the protein levels of the above-mentioned parameters.Results IBS-D related symptoms presented in the mice of the experimental group,such as increased fecal water content and intestinal sensitivity,and shortened intestinal transit time.The expression levels of inflammatory factors(IL-Iβ,IL-6,and TNF-α),NF-κB,TLR4,and MyD88 in the colon tissue of experimental group were significantly higher than those in the control group(P<0.05).Conclusion Intestinal flora inbalance is involved in the development and progression of IBS-D as an initiating important driver.Dysbiosis-induced hypo-inflammation of the intestinal mucosa and abnormal activation of NF-κB-related pathways may play key roles in this process.
