Effect of brain-derived neurotrophic factor/miR-195 on traumatic brain injury
10.3969/j.issn.1009-0754.2018.02.009
- VernacularTitle:脑源性神经生长因子/microRNA-195在 创伤性脑损伤中的作用研究
- Author:
Zhun QU
1
;
Jianhua ZHAO
;
Chao WANG
;
Jun ZHU
Author Information
1. 同济大学医学院
- Keywords:
Brain-derived neurotrophic factor;
miR-195;
Traumatic brain injury;
Apoptosis;
Growth;
Nerve function
- From:
Journal of Navy Medicine
2018;39(2):113-116,120
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of brain-derived neurotrophic factor (BDNF)/miR-195 on traumatic brain injury (TBI).Methods Following admission of the TBI patients and development of the TBI rat model , the levels of BDNF and miR-195 were detected by ELISA and RT-PCR.The relationship between BDNF and miR-195 was detected by Western blotting , and the effects of BDNF and miR-195 on the apoptosis of N2A and SY5Y and on the proliferation of N2A and SY5Y by CCK-8 were identified.And the efficacy of BDNF/miR-195 on TBI was evaluated by NSS score system .Results The serum BDNF level of the TBI patients at admission was significantly lower than that of the healthy group (P<0.05), and the BDNF level in the brain tissue of the TBI rats increased at first and then decreased, and it was obviously lower than the normal level (P<0.05 or P<0.01).The levels of miR-195 in the TBI patients and rats all significantly elevated and were higher than the normal level (P<0.01).BDNF could enhance nerve cell growth and inhibit apoptosis , while miR-195 acted antagonistically to inhibit the growth of nerve cells and promote apoptosis .The effects of BDNF were to promote cell growth and inhibit cell apoptosis by inhibiting the expression of miR -195 and improving the function of nerve cells . Conclusion BDNF/miR-195 could promote nerve cell growth , inhibit apoptosis and improve nerve function , which provided a new tar-get for the treatment of traumatic brain injury .
