Significance of abnormal lipid metabolism induced by hypoxia in mice with pulmonary hypertension
10.3969/j.issn.1009-0754.2018.02.008
- VernacularTitle:低氧环境诱发的脂质代谢异常在肺动脉高压疾病过程中的意义
- Author:
Zhongshuang ZHANG
1
;
Yongbiao SUN
;
Zhaoqian JIA
;
Shouyuan MA
;
Xue ZHOU
;
He QIU
;
Zhiru FAN
;
Ketao MA
;
Hongqiang REN
Author Information
1. 832000 新疆 石河子,石河子大学医学院基础医学系;832000 新疆 石河子,石河子大学医学院新疆地方与民族高发病教育部重点实验室
- From:
Journal of Navy Medicine
2018;39(2):109-112
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the significance of abnormal lipid metabolism induced by hypoxia in mice with pulmonary hypertension.Methods Thirty male C57BL/6 mice were chosen and were randomly divided into the model group and the control group, each consisting of 15 animals.The mice in the model group were exposed to chronic hypoxia for the development of hypoxic pul -monary hypertension model , and the mice in the control group were housed in the chamber at the normal ambient air .Right heart cathe-terization was used to measure right ventricular systolic pressure in the 2 groups, Masson method was used to observe the small pulmona-ry vascular vessel remodeling , and the ELISA method was used to detect levels of high density lipoprotein ( HDL-C) , cholesterol ( TC) and low density lipoprotein (LDL-C).The expressions of HMG-CoA reductase (HMGCR), low density lipoprotein receptor (LDLR) and ATP binding cassette transporter A1 (ABCA1) gene in the liver tissue were detected by real-time quantitative PCR.Results The right ventricular systolic pressure (40.12 ±8.22) mmHg(1 mmHg=0.133 kPa) and right ventricular hypertrophy index (0.352 ± 0.050) in the model group were significantly higher than those in the control group (P<0.05).The pulmonary artery vascular wall of the model group was significantly thicker than that of the control group .The HDL-C level of the model group was (26.20 ±3.73) mg/dl, which was significantly lower than that of the control group (P<0.05).There was no statistical significance in the levels of TC and
