Evaluation of the inhibitive effects of salinomycin and arsenic trioxide on breast cancer cell MCF-7 by using median-effect principle

VernacularTitle:利用中效原理评价盐霉素与三氧化二砷联合对乳腺癌MCF-7细胞的抑制作用
Author: Mingshu ZHOU ¹ ; Shanshan WANG ; Yi WANG ; Shoujun YUAN
Author Information

1. 南华大学附属第二医院泌尿外科
Keywords: Salinomycin; Arsenic trioxide; Median-effect principle; Combined medication
From: Journal of Navy Medicine 2018;39(1):21-24,28
CountryChina
Language:Chinese
Abstract: Objective To study the inhibitive effects of salinomycin in combination with arsenic trioxide(As2O3)on the growth of vitro human breast cancer cell line MCF-7.Methods The inhibitive effects of single drug and combined medication on the cell line MCF-7 were detected by using MTT method.The interactions of the 2 drugs were assessed by using median-effect principle, and the effects of the 2 drugs on cell proliferation and migration were observed by colony and scarification tests,and the effects of single drug medication and combined drug medication on the morphology of MCF-7 apoptosis were observed by Hoechst 33258 staining.Results The effects of single drug medication and combined drug medication increased with the dosage of the drugs.When fa was lower than 0.827 55 and CI was lower than 1,the combined effects of the 2 drugs were synergistic.Conclusion Combined medication of the 2 drugs at low dosage could synergistically inhibit the growth of MCF-7 and could further promote apoptosis.