Effect of CRISPLD2 on the expression of inflammatory cytokine in LPS-induced ARDS mice
10.3969/j.issn.1009-0754.2017.06.010
- VernacularTitle:CRISPLD2蛋白对内毒素-诱导急性呼吸窘迫综合征小鼠炎性因子表达的影响
- Author:
Xuefeng LIU
1
;
Wenfang LI
;
Zhaofen LIN
Author Information
1. 上海长征医院急救科
- Keywords:
rCRISPLD2;
Lipopolysaecharide;
Acute respiratory distress syndrome;
Inflammatory cytokine
- From:
Journal of Navy Medicine
2017;38(6):503-505
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of CRISPLD 2 protein on the expression of inflammatory cytokine in LPS-in-duced acute respiratory distress syndromes ( ARDSs) mice.Methods Forty male Kunming mice were randomly divided into 5 groups;the control group (group A), which was given 0.1mg LPS via the intratracheal instillation;the rCRISPLD2 group 1 (group B) and the rCRISPLD2 group 2 (group C), which were respectively treated with 1mg and 0.4mg rCRISPLD2 by tail vein, and after 6 hours rC-RISPLD2 was injected intratracheally;the rCRISPLD2 group 3 (group D) and the rCRISPLD2 group 4 (group E), which were first given 0.1mg LPS intratracheally and then were injected with 1mg and 0.4mg rCRISPLD2 by tail vein.At hour 12 after intratracheal in-jection of LPS in the mice of all the groups , bronchoalveolar lavage fluid (BALF) was collected.After centrifugation, the number of neutrophils was counted , and the levels of TNF-α, IL-1βand IL-6 were detected in supernatant fluid and blood samples .Results The number of neutrophils(0.31 ±0.07、0.76 ±0.08), TNF-α(8.88 ±17.37、134.63 ±24.44), IL-1β(48.63 ±15.61, 99.75 ±12.30) and IL-6 (80.75 ±21.03,140.12 ±29.29)in serum and BALF in groups B and C were all significantly lower than those of group A , D and E, and statistical significance could be noted , when comparisons were made between these groups (P<0.05).The detected data of group B were obviously lower than those of group C , also with statistical significance (P<0.05).Conclusion rCRISPLD2 could down-regulate the expression levels of LPS-induced inflammatory factors , and dosage dependence could be noted to a certain extent .
