Effects of miRNA-10a on the proliferation of hepatic fibrosis cells and the expression of TGF beta 1/Smads signal transduction pathway in mice
10.3969/j.issn.1009-0754.2017.03.013
- VernacularTitle:miRNA-10a对小鼠肝纤维化细胞增殖及TGFβ1/Smads信号转导通路表达的影响
- Author:
Guosun DENG
1
;
Zhen'ga LI
Author Information
1. 上海交通大学附属新华医院崇明分院消化内科
- Keywords:
Hepatic fibrosis;
Transforming growth factor β1;
Smad 7 protein;
TGFβ1/Smads signal pathway
- From:
Journal of Navy Medicine
2017;38(3):222-225
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the possible mechanism involved in the effect of micro RNA-10a(miRNA) on hepatic fibrosis (HF),through the observation on the expression of TGFβ1/Smads signal transduction pathway in mice with HF and its correlation with the development of hepatic fibrosis.Methods Forty healthy male mice with an age of 9 weeks (C57BL6/J) were randomly divided into the control group and the HF model group (or the observation group).The animals in the control group were given normal saline abdominally at a dosage of 5 μl/g,twice a week,for a succession of 8 weeks,while the animals in the observation group received 10％ CCL4 olive oil also at a dosage of 5 μl/g with the same frequency and for the same length of time to develop the model of hepatic fibrosis.The expression of miRNA-10a was detected by RT-PCR.HF cells were cultured in the animals of the observation group and miRNA-10a mimics were transfected (the transfection group).CCK-8 method was used to detect the proliferation of HF cells,and the expression levels of TGFβ1 and Smad 7 in the HF cells were detected by Western-blotting.Results As compared with that of the control group,the expression level of miRNA-10a in the observation group was increased significantly (P ＜ 0.05);and as compared with that of the control group,the expression level of miRNA-10a in the transfection group were significantly decreased (P ＜ 0.05);and furthermore,as compared with that of the control group,the lower expression of miRNA-10a significantly decreased the expression level of TGFβ1 and increased the expression level of Smad 7 (P ＜ 0.05).Conclusion The expression of miRNA-10a in the HF cells was lower,and the transfected miRNA-10a mimics could significantly promote the proliferation of HF cells,the possible mechanism of which might be associated with the regulation of TGFβ1/Smads signal transduction pathway in HF promotion.
