Determination of the content of menthol in the nasal in-situ gel spray by gas chromatography
10.3969/j.issn.1009-0754.2017.01.009
- VernacularTitle:气相色谱法测定薄荷脑鼻用原位凝胶喷雾剂中薄荷脑的含量
- Author:
Anfeng KANG
1
;
Xinfang LI
;
Lijing LE
;
Jieqiong ZHAN
;
Zhiqiang MA
;
Xinrong ZHANG
;
Feng YANG
Author Information
1. 350122 福州,福建中医药大学;第二军医大学药学院无机化学教研室
- Keywords:
Gas chromatography;
In-situ gel;
Menthol;
Content determination
- From:
Journal of Navy Medicine
2017;38(1):24-26
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for concentration determination of menthol in the nasal in -situ gel spray. Methods Gas chromatography (GC) was performed on the column of Agilent DB-WAX capillary column (30m ×0.25 mm ×0.25μm), with the column temperature of 120 ℃.With nitrogen gas used as carrier gas , hydrogen flame ion detector (FID) was used, the detection temperature was 260℃, injection temperature was 220℃, injection volume was 1μl and column flow rate was 1.0 ml/min. Results For menthol, there was good linear relationship within the range of mass concentration at 0.02~0.20 mg/mL (r=0.9991). Precision, reproducibility and stability test were all lower than 2%, the sample recovery rate was 95.1%~103.4%and relative stand-ard deviation (RSD) was 2.3%(n=6).Conclusion The established method had the following advantages:easy operation, high re-liability and good reproducibility , and it could be used for the content determination of menthol in the nasal in -situ gel spray .
