Protective effect of CRISPLD2 protein on acute respiratory disease syndrome induced by lipopolysaecharide in mice
10.3969/j.issn.1009-0754.2017.01.008
- VernacularTitle:CRISPLD2蛋白对内毒素急性呼吸窘迫综合征小鼠的保护作用
- Author:
Xuefeng LIU
1
;
Jinlong QU
;
Wenfang LI
;
Zhaofen LIN
Author Information
1. 第二军医大学附属长征医院急救科
- Keywords:
CRISPLD2;
Endotoxin;
Lipopolysaecharide;
Acute respiratory disease syndrome
- From:
Journal of Navy Medicine
2017;38(1):21-23
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect of CRISPLD 2 protein on acute respiratory disease syndrome ( ARDS) induced by lipopolysaecharide ( LPS) in mice.Methods Healthy male Kunming mice were randomly divided into 3 groups:the control group (or group A) which received lipopolysaecharide via the intratracheal instillation at a dosage of 5 mg/kg, the LPS pre-conditioning group (or group B) which had intraperitoneal injection of LPS at a dosage of 2mg/kg and at day 8 received LPS via intra-tracheal instillation also at a dosage of 5mg/kg.The recombinant CRISPLD2 preconditioning group ( or group C) which was injected with recombinant CRISPLD2 at a dosage of 50mg/kg via cauda vein, and then at hour 6 received LPS via intratracheal instillation at the same dosage.(1)Thirty mice were randomly divided into group A1, group B1 and group C1, each consisting of 10 animals.The mice were sacrificed 6 hours after LPS intratracheal instillation .Then, W/D and LPI were measured.(2) Ninety mice were randomly divid-ed into group A2, group B2 and group C2, each consisting of 30 animals.The death of the animals in each group was closely observed and the survival curve was described .Results W/D, lung index and mortality after 5 days in group B and C were significantly lower , as compared with those of group A (P<0.05).The detected data of various indexes in group B were lower than those in group C (P>0.05).Conclusion Increased serum concentration of CRISPLD2 could obviously produce protective effect on ARDS induced by LPS , lighten injury on the lungs and improve permeability of pulmonary capillary and decrease mortality .
