Effects of lactoferrin on microglial activation in the spinal cord in the rat model of neuropathic pain
10.3969/j.issn.1009-0754.2016.06.007
- VernacularTitle:乳铁蛋白对神经病理性痛大鼠脊髓小胶质细胞活化的影响研究
- Author:
Jun WANG
1
;
Hong XUE
;
Chuanyue ZONG
;
Yi ZONG
Author Information
1. 淮安市第二人民医院麻醉科
- Keywords:
Lactoferrin;
Neuropathic pain;
Microglia
- From:
Journal of Navy Medicine
2016;37(6):502-504,516
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of lactoferrin on microglial activation in the spinal cord in the rat model of neuropathic pain (NP).Methods Thirty-two male SD rats with the weight of 200-220 g were randomly divided into 4 groups, i.e. the control group , the neuropathic pain group , the minocycline group and the lactoferrin group , each consisting of 8 animals.The sciat-ic nerve was only exposed but not ligated in the animals of the control group , and 8μl of normal saline were injected intrathecally .The neuropathic pain model was produced by placing loosely constrictive ligatures around the common sciatic nerve in the animals of the oth -er groups.Eight μl of normal saline were injected intrathecally in the animals of the neuropathic pain group .One hundred μl of lacto-ferrin were given intrathecally in the animals of the lactoferrin group , while the same amount of minocycline was also given intrathecally in the animals of the minocycline group .Following medication , the paw withdrawal latency ( PWL) was detected every 30 minutes by u-sing thermal nociceptive stimulus with a total of 180 minutes.After detections for 7 times, the rats were sacrificed for the collection of spinal dorsal horn .The expression of Iba-1 was determined by immunofluorescence , and then statistical analyses were made according-ly.Results PWL for the animals of the neuropathic pain group was obviously prolonged , as compared with that of the control group , and statistical significance could be seen , when comparisons were made between them (P<0.05).PWL for the animals of both the mi-
nocycline group and the lactoferrin group was prolonged , as compared with that of the neuropathic pain group , also with statistical signif-icance(P<0.05).No statistical significance could be noted in PWL for the animals of the minocycline group and the lactoferrin group , when comparisons were made between them (P>0.05).This was an indirect indication that lactoferrin could alleviate neuropathic pain through the inhibition of the activation of spinal microglia .The expression levels of Iba-1 in the animals of the minocycline group and the lactoferrin group , as well as the neuropathic pain group , were significantly decreased , and statistical significance could be seen , when it was compared with that of the control group (P<0.05).The expression levels of Iba-1 in the animals of the minocycline group and the lactoferrin group were also significantly decreased , as compared with that of the neuropathic pain group , also with statistical signifi-cance(P<0.05).No statistical significance was shown in PWL , when comparisons were made between the minocycline group and the lactoferrin group(P>0.05).This was also an indirect indication that lactoferrin could alleviate neuropathic pain through the inhibition of the activation of spinal microglia .Conclusion Lactoferrin could alleviate neuropathic pain through the inhibition of the activation of spinal microglia , which had certain medical value in the clinical practice of anesthesia .
