Study on the molecular mechanism of after-cataract induced by mTOR signal pathway and mediated by TGF-β2
10.3969/j.issn.1009-0754.2016.06.006
- VernacularTitle:雷帕霉素靶蛋白信号通路介导转化生长因子-β2诱导的后发性白内障的分子机制研究
- Author:
Rongrong GE
1
;
Wei SHEN
Author Information
1. 解放军第四一一医院眼科
- Keywords:
TGF -β2;
mTOR signal pathway;
Post capsular opacification
- From:
Journal of Navy Medicine
2016;37(6):497-501
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism of the association between mammalian target of rapamycin ( mTOR) sig-nal pathway and TGF-β2-induced lens epithelial mesenchymal transition .Methods Phenotypic changes in lens epithelial cells induced by TGF-β2 were closely observed by microscopy .Western blotting was used to further verify the HLEB 3 epithelial mesenchymal transi-tion model induced by TGF-β2.MTT was applied to detect the effects of TGF-β2 after induction of epithelial mesenchymal and Rapamy-cin on cell proliferation .Western blotting was used to detect the mechanism of the relationship between epithelial mesenchymal transi -tion and mTOR signal pathway at the molecular level .Results After TGF-β2 treatment for 24 hours, microscopic observation revealed that HLEB-3 cells were transformed from the oval shape into the star or fusiform shape , and cell connection was also reduced .Western blotting revealed that the expression levels of epithelial protein marker E-cadherin in HLEB-3, following induction of TGF-β2, was obvi-ously decreased , while the expression levels of the stromal cell protein marker α-SMA were significantly decreased , and statistical sig-nificance could be noticed when comparisons were made between them (P<0.05).MTT test also indicated that Rapamycin pretreat-ment could inhibit the enhancing effect of TGF-β2 on epithelial cell proliferation .Western blotting showed that Rapamycin could reverse the up-regulation of α-SMA by TGF-β2 and promote the expression of epithelial protein marker E-cadherin, following the activation of mTOR signal pathway inhibited by Rapamycin .Conclusion Epithelial mesenchymal transition induced by mTOR signal pathway and
mediated by TGF-β2 could promote the onset of after-cataract.
