Establishment of vitro cell model of human gastric mucosal fibroblasts and preliminary exploration of its biological features
10.3969/j.issn.1009-0754.2016.06.004
- VernacularTitle:体外胃癌间质成纤维细胞模型建立和生物学特性初步研究
- Author:
Hua CHAI
1
;
Hao ZHANG
;
Jianwei BI
Author Information
1. 上海市虹口区江湾医院普外科
- Keywords:
Fibroblast;
Gastric cancer;
Transformation
- From:
Journal of Navy Medicine
2016;37(6):490-492,496
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish vitro cell model of human gastric mucosal fibroblasts ( HGMF) in gastric cancer and ex-plore its biological features .Methods HGMF were cultured with the regulated supernatant of MKN-45 cells, facilitating its transforma-tion.Cell proliferation was detected by MTT assay .The expressions of hepatocyte growth factor ( HGF) , transforming growth factor beta 1(TGF-β1), chemokine ligand 5 (CCL5)and chemokine ligand 9(CXCL9)were detected by ELISA.The activity of telomerase and the ability to synthesize proteins were detected by RT-PCR and BSA assay respectively .Results Supernatant of MKN-45 could induce HGMF transformation.The ability of cell proliferation was enhanced (P<0.05), and the expression levels of HGF, TGF-β1, CCL5 and CXCL9 were also increased (P<0.01).Telomerase activity and the ability to synthesize proteins were also enhanced respectively (P<0.01).Conclusion Detection results suggested that the transformed HGMF seemed to display higher biological activity , as com-pared with that of the parent cells , with stronger proliferation activity and higher protein synthesis , and the HGMF could express and se-crete more growth factor and chemokines associated with the growth of gastric cancer cells .
