The expression of adherent molecules in the preservation of human red blood cells during deployment at sea
10.3969/j.issn.1009-0754.2016.04.016
- VernacularTitle:航海条件下血液不同保存方法对红细胞表达黏附分子的影响
- Author:
Shuxun LIU
1
;
Dahai TANG
;
Jialei HE
;
Lihua HAN
;
Manjiao LIU
;
Jiang WU
Author Information
1. 第二军医大学免疫学教研室
- Keywords:
Shipboard blood transport;
Blood preservation;
Erythrocyte;
Adherent molecule
- From:
Journal of Navy Medicine
2016;37(4):332-334
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of blood-bag placement mode in the refrigerator on the expression of adherent molecules in the preservation of human erythrocytes onboard the ship.Methods On the first day of deployment to sea, 4 blood samples were collected from 4 healthy volunteers.Erythrocyte suspension was equally aliquoted into four portions, one as the control group was placed vertically in the refrigerator of the hospital and the other three as the experiment group were preserved in the same refrigerator on-board the ship in 3 different placement modes ( the layer-upon-layer mode, the upright mode and the vertical suspension mode) .After completion of the training, the preserved samples were immediately sent to the laboratory for the detection of the expressions of CD47, CD58, and CD44.Results The expression levels of CD44 and CD47 in the erythrocytes of whole blood placed on the shipboard refrig-erator were significantly higher, as compared with those of the control group in the hospital.The expression levels of CD58 in the eryth-rocytes placed in the layer-upon-layer mode decreased significantly.Conclusion From the aspect of the expression of erythrocyte, the upright placement mode was most suitable for the preservation of blood in the shipboard refrigerator.
