Role of Toll-like receptor 4 expression inhibition in the transformation of macrophage polarity
10.3969/j.issn.1009-0754.2015.03.006
- VernacularTitle:抑制 Toll样受体4表达对巨噬细胞极性转化的影响
- Author:
Zhenli ZHAI
1
;
Wei SHEN
;
Weihong MA
;
Qinghai JIAO
Author Information
1. 邯郸市第一医院重症医学科
- Keywords:
Toll-like receptor 4;
Macrophage;
Macrophage polarity
- From:
Journal of Navy Medicine
2015;(3):209-212
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of Toll-like receptor 4 in the transformation of the bone marrow-derived macrophage polarity through observation on the effects of CLI-095 on phenotype of macrophages.Methods Our research subjects were the cultured mouse marrow-derived macrophages, and were randomly divided into three groups:the control group ( marrow-derived macrophages cul-tured for 48 h without any drug treatment), the model group (marrow-derived macrophages cultured routine for 24 h, then, addition of 100 U/mlγinterferon and 5 ng/ml lipopolysaccharide into the culture medium and cultured for another 24 h) and the treatment group( first in-cubated in 1 μg/ml CLI-095 for 24 h, then, after change of the fluid, addition of 100 U/mlγinterferon and 5 ng/ml lipopolysaccharide into the culture medium and cultured for another 24 h) .Real-time quantitative PCR was used to detect the mRNA expression of TLR4. The expression of membrane molecules CD16/32, CD206 was detected by using fluorescence activated cell sorting (FACS), and enzyme linked immunosorbent assay (ELISA) was used to detect the secretion of interleukin-10 (IL-10) and IL-12.Results As compared with those of the control group, the expression levels of CD16/32 and IL-12 in the model group were increased significantly, and the level of CD206 was decreased markedly, which was in conformity with the features of macrophages.When compared with those of the model group, the level of TLR4 mRNA was decreased.This indicated that the expression level of TLR4 was inhibited, the levels of CD16/32 and IL-12 were decreased and the levels of CD206 and IL-10 were increased with statistical significance and they were also in conformity with the features of macrophages.Conclusion TLR4 seemed to play an important role in the modulation of macrophage polarity.The inhibited expression level of TLR4 could promote inflammatory macrophages towards an anti-inflammatory M2 phenotype.
