Effects of Compound Huangdai Tablets on Necroptosis in an Animal Model of Acute Promyelocytic Leukemia
10.13359/j.cnki.gzxbtcm.2025.11.027
- VernacularTitle:复方黄黛片对急性早幼粒细胞白血病动物模型坏死性凋亡的影响
- Author:
Yueyang ZHAO
1
;
Yuanbin WU
Author Information
1. 广州中医药大学第二临床医学院,广东 广州 510120;广东省中医院血液科,广东 广州 510120
- Keywords:
Compound Huangdai Tablets;
acute promyelocytic leukemia(APL);
necroptosis;
MLKL;
nude mice
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2025;42(11):2816-2822
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the therapeutic effects and mechanism of Compound Huangdai Tablets on nude mice with acute promyelocytic leukemia(APL).Methods An APL xenograft model was established in nude mice using NB4 cells.Successfully modeled mice were divided into a control group and low-and high-dose Compound Huangdai Tablets groups.The grouping intervention was as follow:the low-and high-dose Compound Huangdai Tablets groups were administered Compound Huangdai Tablets solution(suspended in 0.5％sodium carboxymethyl cellulose solution)by gavage at doses of 545 and 1 090 mg·kg-1,respectively,while the control group received an equal volume of normal saline.The administration was performed twice daily,with each dose being 0.2 mL,for a total duration of 25 days.The general condition,tumor volume,and tumor weight were observed.Reactive oxygen species(ROS)levels in tumor tissues were measured.Western Blot and immunohistochemistry(IHC)were used to detect the expression levels of key necroptosis molecules,including mixed lineage kinase domain-like protein(MLKL),receptor-interacting protein kinase 1(RIPK1),and receptor-interacting protein kinase 3(RIPK3).Results Mice in the Compound Huangdai Tablets groups showed no abnormal mental status,sudden weight loss,or feeding difficulties.There was no significant difference in body mass among the groups(P＞0.05).Compared with the control group,the high-dose Compound Huangdai Tablets group showed significantly reduced tumor volume and mass(P＜0.05),along with increased ROS levels in tumor tissues(P＜0.05).The expression levels of MLKL,RIPK1,and RIPK3 proteins in tumor tissues of the high-dose group were higher than those in the control group(P＜0.05).Conclusion Compound Huangdai Tablets promote necroptosis in nude mice with acute promyelocytic leukemia by activating MLKL protein.
