Anti-Hyperuricemic and Anti-Gouty Arthritis Effects of Xiezhuo Qutong Formula and Its Network Pharmacology Mechanism
10.13359/j.cnki.gzxbtcm.2025.11.026
- VernacularTitle:泄浊祛痛方抗高尿酸血症及抗痛风性关节炎的作用及网络药理学机制
- Author:
Shulin CHEN
1
;
Lianjie LIU
;
Xiaolu GAO
;
Xuehong KE
;
Changsong LIN
Author Information
1. 中医药广东省实验室,广东珠海 519031;广州中医药大学,广东 广州 510006
- Keywords:
Xiezhuo Qutong Formula;
gout;
uric acid reduction;
anti-inflammation;
network pharmacology;
rats
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2025;42(11):2807-2816
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the therapeutic effects and mechanism of Xiezhuo Qutong Formula on gout.Methods(1)Animal experiments:A hyperuricemia(HUA)model was established in SD rats using hypoxanthine combined with potassium oxonate,and an acute gouty arthritis(AGA)model was induced by monosodium urate.The efficacy and safety of Xiezhuo Qutong Formula in reducing serum uric acid levels and exerting anti-inflammatory effects were evaluated.(2)Network pharmacology:The main active components and potential targets of Xiezhuo Qutong Formula were collected,along with gout-related disease targets.The intersection of these targets yielded potential therapeutic targets.A protein-protein interaction(PPI)network was constructed,followed by Gene Ontology(GO)functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis.Results Xiezhuo Qutong Formula effectively reduced serum uric acid levels in HUA model rats without observed hepatorenal toxicity.It also decreased joint inflammation index,joint swelling degree,and inflammatory cytokine levels in AGA model rats.Network pharmacology analysis identified 156 overlapping targets between the drug and disease,among which TP53,STAT3,PIK3CA,BCL2,PIK3CD,PIK3CB,JUN,HDAC1,ESR1,and RELA may be key targets of Xiezhuo Qutong Formula in treating gout.Combined with GO and KEGG enrichment analyses,the main involved pathways include PI3K/AKT,JAK2/STAT3,and NF-κB signaling pathways.Conclusion Xiezhuo Qutong Formula exerts uric acid-lowering and anti-inflammatory effects in the treatment of gout,and its potential mechanism involves the PI3K/AKT,JAK2/STAT3,and NF-κB signaling pathways.
