Effects of Buyang Huanwu Decoction on the Sirt1/FOXO3/Wnt/β-catenin Pathway in a Rat Model of Lumbar Disc Herniation
10.13359/j.cnki.gzxbtcm.2025.10.027
- VernacularTitle:补阳还五汤对腰椎间盘突出症模型大鼠Sirt1/FOXO3/Wnt/β-catenin通路的影响
- Author:
Ping LI
1
;
Xin XIAO
Author Information
1. 武汉市中医医院骨科,湖北武汉 430074
- Keywords:
Buyang Huanwu Decoction;
lumbar disc herniation;
inflammatory pain(LDH);
Sirt1;
FOXO3;
Wnt;
β-catenin;
rats
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2025;42(10):2549-2555
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the therapeutic effects and mechanism of Buyang Huanwu Decoction(BYHWD)in rats with lumbar disc herniation(LDH).Methods An LDH model was established in rats using autologous nucleus pulposus transplantation.After modeling,the rats were randomly divided into four groups(n=12 each):model group,BYHWD group,EX-527(Sirt1 inhibitor)group,and BYHWD+EX-527 group,with an additional sham-operated group(n=12).After two weeks of treatment,pain behaviors were assessed by measuring mechanical withdrawal threshold(MWT)and paw withdrawal thermal latency(PWTL).Serum levels of pain mediators(5-HT,NPY)and inflammatory cytokines(TNF-α,IL-1β,IL-6)were quantified via ELISA.Histopathological changes in lumbar tissues were evaluated by hematoxylin-eosin(HE)staining,while protein expression of Sirt1,FOXO3,Wnt,and β-catenin was detected by Western Blot.Results Compared with the sham group,the model group exhibited:decreased MWT,PWTL,serum 5-HT,and lumbar Sirt1/FOXO3 expression(P＜0.05);elevated serum NPY,IL-6,IL-1β,TNF-α,and lumbar Wnt3a/β-catenin levels(P＜0.05);and pathological features including swollen nucleus pulposus cells,blurred annular boundaries,torn/disorganized collagen fibers,and increased inflammatory infiltration.Compared with the model group,BYHWD group exhibited increased MWT,PWTL,5-HT,and Sirt1/FOXO3(P＜0.05);reduced NPY,IL-6,IL-1β,TNF-α,and Wnt3a/β-catenin(P＜0.05);and improved histopathology with partial nucleus pulposus atrophy,clearer annular borders,and reduced inflammation.EX-527 exacerbated hyperalgesia and attenuated BYHWD's analgesic effects(P＜0.05),with statistically significance.Conclusion BYHWD alleviates inflammatory pain in LDH rats by upregulating Sirt1,enhancing FOXO3 expression,and suppressing Wnt/β-catenin pathway activation,thereby reducing cytokine release and improving pain thresholds.
