Effects of Herbal Compatibility on Chemical Composition and Neuroinflammatory Activity of Banxia Houpo Decoction
10.13359/j.cnki.gzxbtcm.2025.09.030
- VernacularTitle:中药配伍对半夏厚朴汤化学成分及神经炎症作用的影响
- Author:
Yuanning ZENG
1
;
Yaming CHEN
;
Huilin SU
;
Qiuhong WANG
;
Qian WANG
Author Information
1. 广东药科大学中药学院,广东 广州 510006
- Keywords:
Banxia Houpo Decoction;
herbal compatibity;
neuroinflammation;
neuronal injury;
honokiol;
magnolol;
UPLC-MS;
BV2 cells;
SH-SY5Y cells
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2025;42(9):2305-2313
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of herbal compatibility on the chemical composition of Banxia Houpo Decoction(BHD)using ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS)coupled with multivariate statistical analysis,and to evaluate the neuroprotective effects of key differential components against neuroinflammation and neuronal injury using cellular models.Methods(1)UPLC-MS analysis of chemical constituents in co-decoction and separated decoction(individual herbs decocted separately then combined)of Banxia Houpo Decoction,followed by orthogonal partial least squares-discriminant analysis(OPLS-DA)to identify differential components before and after herbal compatibility(2)BV2 microglia were stimulated with lipopolysaccharide(LPS)to establish a neuroinflammation model.Cell viability was assessed using the Cell Counting Kit 8(CCK-8)assay.Nitric oxide(NO)levels were measured by the Griess method,while TNF-α and IL-1β concentrations were quantified via enzyme-linked immunosorbent assay(ELISA).(3)SH-SY5Y neuronal cells were co-cultured with conditioned medium from LPS-stimulated BV2 cells(LPS-CM)to model neuronal injury.Cell viability was evaluated using the CCK-8 assay.Results UPLC-MS/OPLS-DA identified 11 differential components between compatibility methods,with honokiol and magnolol showing significant post-compatibility increases.In the neuroinflammation model,LPS stimulation elevated NO,TNF-α and IL-1 β levels in BV2 cells,which were suppressed by 5,10 μg/mL honokiol or magnolol.In the neuronal injury model,LPS-CM induced SH-SY5Y apoptosis,while 5,10 μg/mL honokiol or magnolol attenuated this damage.Conclusion Herbal compatibility significantly enhances honokiol and magnolol content in BHD.These components inhibit microglial inflammatory responses and neuronal apoptosis,suggesting their role as primary active constituents mediating BHD's neuroprotective effects.
