Establishment of Human Luminal Breast Cancer Stem Cell Model and the Therapeutic Effects of Astragaloside Ⅳ
10.13359/j.cnki.gzxbtcm.2025.09.029
- VernacularTitle:人源Luminal型乳腺癌干细胞模型构建及黄芪甲苷的干预作用
- Author:
Liushan CHEN
1
;
Huachao LI
;
Yingchao WU
;
Yuqi LIANG
;
Peng WU
;
Congwen YANG
;
Junfeng HUANG
;
Jieting CHEN
;
Zhili ZENG
;
Chen FANG
;
Qian ZUO
;
Qianjun CHEN
Author Information
1. 中医药广东省实验室,广东珠海 519031;广州中医药大学第二附属医院中医证候全国重点实验室,广东 广州 510120;广东省中医院乳腺科,广东 广州 510120;广东省中医药科学院,广东 广州 510120
- Keywords:
astragaloside Ⅳ(AS-Ⅳ);
breast cancer;
cell cycle;
cellular senescence;
stem cell model;
MCF7 cells
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2025;42(9):2295-2304
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a human luminal breast cancer stem cell(BCSC)model and investigate the inhibitory effects of astragaloside Ⅳ(AS-Ⅳ)on BCSC growth.Methods MCF-7 breast cancer cells were cultured in stem cell-specific medium to induce BCSC formation.The BCSCs were then divided into a blank control group and an AS-Ⅳ treatment group,both groups were given PBS or AS-Ⅳ treatment.Morphological changes were observed after intervention.The therapeutic efficacy of AS-Ⅳ was evaluated using 3D spheroid formation and cell viability assays.Transcriptomic profiling and gene expression analysis were performed to elucidate the underlying mechanisms.Results Compared with the MCF7 breast cancer cells,MCF7 breast cancer stem cell mammospheres exhibited accelerated growth(P＜0.01)and significantly increased expression of the stemness marker ALDH1A1(P＜0.01).Further comparison with the blank control group revealed that astragaloside Ⅳ(AS-Ⅳ)treatment significantly inhibited MCF7 breast cancer stem cell proliferation(P＜0.001)and slowed mammosphere growth(P＜0.01).Transcriptomic analysis demonstrated that differentially expressed genes(DEGs)induced by stem cell modeling and AS-Ⅳ intervention were enriched in the cellular senescence signaling pathway.AS-Ⅳ intervention substantially increased the number of SA-β-gal-positive cells(P＜0.01).RT-PCR analysis confirmed that AS-Ⅳsignificantly upregulated mRNA expression of IL-1α(P＜0.01),P21(P＜0.001),and P53(P＜0.05)in MCF7 breast cancer stem cells.Conclusion Astragaloside Ⅳ suppresses the growth of human luminal breast cancer stem cells by inducing cellular senescence.
