Optimization of Kaempferol Extraction from Alpinia officinarum Hance Using Box-Behnken Response Surface Methodology and Evaluation of Its Antioxidant Activity
10.13359/j.cnki.gzxbtcm.2025.08.030
- VernacularTitle:Box-Behnken响应面法优化高良姜中山奈酚提取工艺及其抗氧化活性研究
- Author:
Zhenying FENG
1
;
Qiaofeng LI
;
Minqi DENG
;
Jingyi WU
;
Yingying HE
;
Liping HUANG
Author Information
1. 广东医科大学附属第二医院,广东 湛江 524001
- Keywords:
Alpinia officinarum Hance;
kaempferol;
high-performance liquid chromatography(HPLC);
extraction process;
antioxidant activity
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2025;42(8):2034-2042
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the optimal extraction process for kaempferol from Alpinia officinarum Hance and to investigate its antioxidant activity.Methods Based on single-factor experimental data,this study employed the Box-Behnken central composite design and response surface methodology(RSM),combined with high-performance liquid chromatography(HPLC),to plot response surface and contour maps using kaempferol content as the response value.The optimal parameters for ethanol concentration,solid-liquid ratio,extraction time,and extraction temperature were selected.The antioxidant activity of kaempferol was comprehensively evaluated by calculating the radical scavenging rate using the DPPH radical scavenging assays.Results The optimal extraction conditions were determined to be an ethanol concentration of 84%,a solid-liquid ratio of 1∶80,an extraction time of 2 hours,and an extraction temperature of 82℃.Under these conditions,the kaempferol content extracted from Alpinia officinarum Hance reached 0.034 16%.In the DPPH radical scavenging assay,the extract showed a scavenging rate of 91.20%at a concentration of 1 mg/mL.Conclusion The optimized extraction process for kaempferol from Alpinia officinarum Hance was obtained using the Box-Behnken response surface design method,and this process is practical and feasible.The DPPH radical scavenging assay demonstrated that kaempferol from Alpinia officinarum Hance possesses antioxidant activity.
