Exploring the Reparative Effect of Astragaloside Ⅳ on Hypoxia-Reoxygenation-Induced Cardiomyocyte Injury via the TXNIP/NLRP3 Pathway
10.13359/j.cnki.gzxbtcm.2025.08.027
- VernacularTitle:基于TXNIP/NLRP3通路探讨黄芪甲苷对缺氧复氧诱导心肌细胞损伤的修复作用
- Author:
Lin SHI
1
;
Jiankun WU
;
Juju SHANG
Author Information
1. 首都医科大学附属北京中医医院药学部,北京 100010
- Keywords:
astragaloside Ⅳ;
myocardial ischemia/reperfusion injury(MI/RI);
hypoxia-reoxygenation;
thioredoxin-interacting protein-Nod-like receptor protein 3 pathway;
cardiomyocytes
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2025;42(8):2015-2021
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the reparative effect of astragaloside IV on hypoxia-reoxygenation-induced cardiomyocyte injury by regulating the thioredoxin-interacting protein(TXNIP)-Nod-like receptor protein 3(NLRP3)pathway.Methods H9c2 cardiomyocytes were divided into control group,hypoxia-reoxygenation group,astragaloside IV low-and high-dose groups,astragaloside IV high-dose+pcDNA-NC group,and astragaloside IV high-dose+pcDNA-TXNIP group.After intervention,H9c2 proliferation was measured by Cell Counting Kit 8(CCK-8)assay,apoptosis was detected by flow cytometry,lactate dehydrogenase(LDH)and superoxide dismutase(SOD)activities were determined by WST-8 assay,probebyreactive oxygen species(ROS)generation was measured by 2',7'-dichlorodihydrofluorescein diacetate(DCFDA)fluorescent,enzyme-linked inmunosorbent assay(ELISA)was used to quantify interleukin 6(IL-6)and tumor necrosis factor α(TNF-α)levels,Western Blot was used to analyze the protein expression of TXNIP and NLRP3.Results Compared with the control group,the hypoxia-reoxygenation group showed significantly decreased cell survival rate and SOD activity(P＜0.05),with increased apoptosis rate,LDH level,ROS generation,IL-6 and TNF-α levels,and protein expression levels of TXNIP and NLRP3(P＜0.05).Compared with the hypoxia-reoxygenation group,the cell survival rate and SOD activity in the low-dose and high-dose astragaloside IV groups were significantly increased(P＜0.05),and the apoptosis rate,LDH level,ROS production,IL-6 and TNF-α levels and protein expression levels of TXNIP and NLRP3 were significantly decreased(P＜0.05).Compared with the high-dose astragaloside IV+pcDNA-NC group,the cell survival rate and SOD activity in the high-dose astragaloside IV+pcDNA-TXNIP group were significantly decreased(P＜0.05),and the apoptosis rate,LDH level,ROS production,IL-6 and TNF-α levels and protein expression levels of TXNIP and NLRP3 were significantly increased(P＜0.05).Conclusion Astragaloside IV may alleviate hypoxia-reoxygenation-induced cardiomyocyte damage by downregulating key proteins in the TXNIP-NLRP3 pathway.
