Huangqi Jianzhong Decoction Ameliorates Helicobacter pylori-Induced Gastric Mucosal Injury in Rats by Suppressing the IRF8/IFN-γ Signaling Pathway
10.13359/j.cnki.gzxbtcm.2025.07.029
- VernacularTitle:黄芪建中汤抑制IRF8/IFN-γ信号通路改善幽门螺杆菌感染诱导的大鼠胃黏膜损伤
- Author:
Lijie YU
1
;
Tao LIU
;
Zhongwei SHEN
;
Biwen ZHANG
;
Ke WANG
Author Information
1. 南京中医药大学,江苏 南京 210000;无锡市第二中医医院消化科,江苏 无锡 214000
- Keywords:
Huangqi Jianzhong Decoction;
Helicobacter pylori infection;
gastric mucosal injury;
IRF8/IFN-γ signaling pathway;
inflammatory response;
rats
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2025;42(7):1757-1764
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the therapeutic effects and mechanisms of Huangqi Jianzhong Decoction(HJD)on gastric mucosal injury induced by Helicobacter pylori(Hp)infection.Methods A Wistar rat model of Hp-induced gastric mucosal injury was established.Successfully modeled rats were randomly divided into five groups:model group,low-,medium-and high-dose HJD groups,and quadruple therapy group(Omeprazole+Amoxicillin+Clarithromycin+Colloidal Bismuth pectin),8 rats per group,with an additional normal group.After 4 weeks of treatment,gastric mucosal pathological changes were assessed by hematoxylin-eosin(HE)staining.Serum levels of interleukin 1β(IL-1β),interleukin 18(IL-18),tumor necrosis factor α(TNF-α),chemokine(C-X-C motif)ligand 1(CXCL1),chemokine(C-X-C motif)ligand 9(CXCL9),and gastrin-17(G-17)were measured by enzyme-linked immunosorbent assay(ELISA).Protein expression levels of IRF8,IFN-γ,interferon-induced tetratricopeptide repeat protein 3(Ifit3)and uridine phosphorylase 1(Upp1)in gastric mucosal tissues were detected by Western Blot.Results Compared with the normal group,the model group exhibited significant gastric mucosal damage,serum levels of IL-1β,IL-18,TNF-α,CXCL1 and CXCL9 were significantly increased,G-17 was drcreased,and protein expression levels of IRF8,IFN-γ,Ifit3 and Upp1 in gastric mucosa were significantly increased,the differences being statistically significant(P＜0.05).Compared with the model group,the gastric mucosal tissue injury of rats in the medium-and high-dose of HJD groups and the quadruple therapy group was significantly improved,the levels of IL-1β,IL-18,TNF-α,CXCL1 and CXCL9 in serum were significantly decreased,G-17 was significantly increased,and the protein expression levels of IRF8,IFN-γ,Ifit3 and Upp1 in gastric mucosa were significantly decreased,the differences being statistically significant(P＜0.05).The effect in above indexes in high-does HJD groups was superior to the low-and mediun-groups.Conclusion HJD alleviates Hp-induced gastric mucosal injury by inhibiting the IRF8/IFN-γ signaling pathway and subsequent inflammatory responses.
