Mechanism of Dingkun Dan in Promoting Ovarian Granulosa Cell Proliferation in Polycystic Ovary Syndrome via microRNA-30d-5p-Mediated Targeting of Smad2
10.13359/j.cnki.gzxbtcm.2025.07.028
- VernacularTitle:定坤丹通过microRNA-30d-5p靶向调节Smad2促进多囊卵巢综合征卵巢颗粒细胞增殖的机制研究
- Author:
Lan CHEN
1
;
Xiaojing YAN
;
Yuanpei LIAN
;
Li ZHU
;
Shikang ZHOU
;
Dijun WANG
;
Jiali CAI
;
Chunyan YIN
;
Ji CAO
Author Information
1. 常州市中医医院孟研所,江苏 常州 213003
- Keywords:
Dingkun Dan;
polycystic ovary syndrome(PCOS);
transforming growth factor β1;
Smad2;
microRNA-30d-5p;
ovarian granulosa cells;
rats
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2025;42(7):1750-1756
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the therapeutic mechanism of Dingkun Dan(DKD)in polycystic ovary syndrome(PCOS)by examining the effects of microRNA-30d-5p on ovarian granulosa cells(GCs).Methods A PCOS rat model was established using dehydroepiandrosterone(DHEA).Normal rat GCs and PCOS rat GCs were cultured in vitro and divided into four groups:blank control group,model group,low-dose DKD group,and high-dose DKD group.After grouping,GCs viability was assessed using the methyl thiazolyl tetrazolium(MTT)assay,GCs apoptosis was analyzed by flow cytometry,and the gene expression of transforming growth factor β1(TGF-β1),Smad2,Smad3,and microRNA-30d-5p in GCs was measured by real-time polymerase chain reaction(RT-PCR),protein expression of TGF-β1,Smad2,and Smad3 in GCs was detected by Western Blot.Results Compared with the blank control group,the model group exhibited significantly decreased GCs viability,increased GCs apoptosis,upregulated mRNA and protein expression of TGF-β1,Smad2,and Smad3,and downregulated microRNA-30d-5p expression,the differences were statistically significant(P＜0.01).Compared with the model group,both low-and high-dose DKD groups showed increased GCs viability,reduced GCs apoptosis,downregulated mRNA and protein levels of TGF-β1 Smad2 and Smad3,elevated microRNA-30d-5p expression,and the differences were statistically significant(P＜0.05 or P＜0.01).Conclusion DKD promotes GCs proliferation by targeting Smad2 via microRNA-30d-5p,suggesting a potential therapeutic role in PCOS-related ovulatory dysfunction.
