Exploring the Mechanism of Danggui Shaoyao San in Treating AM,EMs and SPID via the"Same Treatment for Different Diseases"Principle Based on the Network Pharmacology and Molecular Docking
10.13359/j.cnki.gzxbtcm.2025.07.026
- VernacularTitle:基于网络药理学及分子对接探讨当归芍药散"异病同治"子宫腺肌病、子宫内膜异位症与盆腔炎性疾病后遗症的作用机制
- Author:
Tingting HOU
1
;
Yanfeng LIU
;
Ying LI
;
Zhibo ZHENG
Author Information
1. 北京中医药大学东直门医院妇科,北京 100700
- Keywords:
Danggui Shaoyao San;
adenomyosis(AM);
endometriosis(EMs);
sequelae of pelvic inflammatory disease(SPID);
same treatment for different diseases;
network pharmacology;
molecular docking
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2025;42(7):1733-1743
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanisms of Danggui Shaoyao San(DSS)in treating adenomyosis(AM),endometriosis(EMs),and sequelae of pelvic inflammatory disease(SPID)through network pharmacology and molecular docking,guided by the traditional Chinese medicine(TCM)principle of"same treatment for different diseases".Methods Chemical components of DSS were retrieved from the TCMSP and SwissTargetPrediction databases,and their targets were identified.Disease targets for AM,EMs,and SPID were collected from DrugBank,OMIM,GeneCards,and DisGeNET.A Venn diagram was constructed using Venny 2.1 to identify common targets between DSS and the diseases.A"drug-active component-shared target"network was established via Cytoscape 3.7.2.Protein-protein interaction(PPI)networks were analyzed using STRING and Cytoscape 3.7.2 to explore molecular mechanisms.Key targets were localized to tissues using BioGPS.Functional enrichment analysis of GO terms and KEGG pathways was performed via DAVID,followed by molecular docking validation.Results Thirty-nine active components and 529 potential targets of DSS were identified,with 60 shared targets across the three diseases.Enrichment analysis revealed that DSS treats AM,EMs and SPID by modulating cancer-related pathways,the PI3K/Akt signaling pathway,HIF-1 signaling pathway,and TNF signaling pathway.Molecular docking demonstrated stable binding conformations between DSS's primary active components and core targets.Conclusion DSS treats AM,EMs and SPID through multiple compounds[e.g.,(+)-catechin,kaempferol,β-sitosterol]acting on key targets(TNF,EGFR,PTGS2,HIF1A)across various organs,modulating inflammation,immune response,angiogenesis,and cell signaling pathways,thereby exerting its"same treatment for different diseases"effect.
