Analysis of Gut Microbiota Diversity in Osteoporosis Patients with Various Traditional Chinese Medicine Syndrome Types in the Lingnan Region
10.13359/j.cnki.gzxbtcm.2025.07.004
- VernacularTitle:岭南地区骨质疏松患者不同中医证型肠道菌群多样性分析
- Author:
Huan LI
1
;
Wending YAN
;
Tianzhao TIAN
;
Jinyuan WANG
;
Haodong LIANG
Author Information
1. 广州中医药大学附属广州中医医院,广东 广州 510130
- Keywords:
Lingnan region;
osteoporosis;
gut microbiota;
traditional Chinese medicine(TCM)syndrome types;
blood stasis and qi stagnation syndrome;
liver-kidney deficiency syndrome;
microbial diversity analysis
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2025;42(7):1574-1582
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the diversity of gut microbiota in osteoporosis(OP)patients with various traditional Chinese medicine(TCM)syndrome types in the Lingnan region.Methods The analysis was carried out in 13 OP patients with blood stasis and qi stagnation syndrome(Group 1)and 13 OP patients with liver-kidney deficiency syndrome(Group 2)who were treated at Guangzhou Hospital of Chinese Medicine,the Affiliated Hospital of Guangzhou University of Chinese Medicine from January 2023 to December 2023.Fecal samples of both groups were collected,and baseline comparisons between the two groups were performed before testing.After DNA extraction,valid sequencing data were processed by operational taxonomic unit(OTU)clustering,species annotation,and microbial community composition analysis.Alpha and Beta diversity indices were employed to assess species diversity,and LEfSe was used to identify key microbial taxa.Results No significant differences in baseline characteristics such as gender and age were presented between the two groups(P＞0.05).Microbial community composition analysis revealed no significant differences in the top 15 abundant taxa at various taxonomic levels between the two groups,but structural variations were noted.Alpha diversity analysis showed that the Simpson index in Group 1 differed from that in Group 2(P＜0.05),while chao 1,ACE,richness,and coverage indices exhibited no significant differences between the two groups(P＞0.05).In Beta diversity analysis,multi-response permutation analysis(MRPP)indicated no significant intergroup differences in community structure(P＞0.05),whereas principal coordinate analysis(PCoA)suggested distinct differences in the distribution of species composition.LEfSe identified 14 taxa(e.g.,Lachnospiraceae and Lachnospirales)with significantly higher abundance and 5 taxa(e.g.,Anaeromassilibacillus and Desulfovibrio)with significantly lower abundance in Group 1.Conclusion OP patients with various TCM syndrome types in the Lingnan region exhibit distinct gut microbiota diversity and dominant microbial taxa,which will provide an objective basis for TCM syndrome differentiation of OP.
