Transcriptome Sequencing and Bioinformatics Analysis of Herba Chlorophyti Laxi
10.13359/j.cnki.gzxbtcm.2025.06.027
- VernacularTitle:三角草转录组测序及生物信息学分析
- Author:
Jingyu LI
1
;
Yiheng DAI
;
Yan GU
;
Xuqiang LUO
;
Yu MEI
;
Jihua WANG
Author Information
1. 广东省农作遗传改良重点实验室 广东省农业科学院作物研究所,广东 广州 510640;广东省道地南药资源保护与利用工程中心,广东 广州 510640
- Keywords:
Chlorophtum laxum R.Br.;
active component;
transcriptome;
unigene;
functional annotation;
SSR
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2025;42(6):1485-1491
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the transcriptional expression of Chlorophyti Laxi R.Br.using high-throughput sequencing technology.Methods Total RNA of Chlorophtum laxum R.Br.was extracted,followed by library construction,sequencing,and de novo assembly to obtain unigene sequences.These sequences were then annotated and compared to acquire genetic information of the Chlorophtum laxum R.Br.transcriptome.Results A total of 29 325 unigene were identified,with an average length of 1 026 bp and an N50 of 1 697 bp.Among them,20 338 unigene were functionally annotated in at least one database,with Chlorophtum laxum R.Br.showing the highest sequence similarity to Asparagus officinalis.In the Kyoto Encyclopedia of Genes and Genomes(KEGG)database,13 392 unigene were annotated,including genes involved in flavonoid biosynthesis and ubiquinone and other terpenoid-quinone biosynthesis pathways.Thirty-five upstream genes related to Chlorophtum laxum R.Br.saponin biosynthesis were identified.In the Gene Ontology(GO)database,16 728 unigene were annotated,covering cellular anatomical entities,binding,and biological processes.Using the Microsatellite Identification Tool(MISA),11 486 assembled unigene longer than 1 000 bp were analyzed for simple sequence repeats(SSRs),resulting in the identification of 5 178 SSR loci,for which primers were designed using Primer 3.0.By comparing Chlorophtum laxum R.Br.unigene with the transcription factor database,657 transcription factors,including bHLH,MYB,and WRKY,were identified.Conclusion The transcriptome data provide a foundation for studying the biosynthetic pathways and functional genes of medicinal active components in Chlorophtum laxum R.Br.,and also contribute to the conservation and development of its resources.
