Study on Thin-Layer Identification of Key Herbal Medicines in a Medicinal Bath Formula for Treating Inflammatory Fever Based on IL-1β Regulatory Activity and Content Determination of Index Components
10.13359/j.cnki.gzxbtcm.2025.06.026
- VernacularTitle:基于IL-1β调控活性的外感发热药浴方治疗炎性发热功效主要药味薄层鉴别和指标成分的含量测定研究
- Author:
Genxiang HE
1
;
Meiying TAN
;
Liangying LIN
;
Jingsong ZHOU
;
Shaoting CEN
;
Minyu WANG
Author Information
1. 佛山市三水区妇幼保健院药剂科,广东 佛山 528100
- Keywords:
medicinal bath formula for exogenous fever;
IL-1β;
thin-layer identification;
index component;
content determination
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2025;42(6):1478-1484
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a quality standard for the medicinal bath formula for treating exogenous fever(composed of Forsythiae Fructus,Peucedani Radix,Schizonepetae Spica,Isatidis Radix,Gypsum Fibrosum,Chrysanthemi Flos,Lophatheri Herba,etc.)based on its efficacy and indications.Methods Thin-layer chromatography(TLC)was used to establish identification method for the monarch drugs Forsythiae Fructus and Peucedani Radix.Enzyme-linked immunosorbent assay(ELISA)was employed to measure the interleukin 1β(IL-1β)regulatory activity of the main components in the formula.High-performance liquid chromatography(HPLC)was used to determine the content of active components in 10 batches of samples.Results Spots of Forsythiae Fructus and Peucedani Radix were successfully detected in the test samples.ELISA identified active components in the formula,including praeruptorin A,pulegone,rutin,praeruptorin B,forsythoside,and(R,S)-goitrin.The content determination results of 10 batches of samples showed that the content of praeruptorin A ranged from 0.493 to 0.694 mg·mL-1.Conclusion Based on its efficacy and indications,TLC identification and HPLC content determination methods were established for the medicinal bath formula for treating exogenous fever.The obtained standard can more accurately control the efficacy of the formula.
