Asiaticoside Alleviates Renal Fibrosis in Diabetic Nephropathy Rats by Regulating the Hippo-YAP/TAZ Signaling Pathway
10.13359/j.cnki.gzxbtcm.2025.06.025
- VernacularTitle:积雪草苷通过调节Hippo-YAP/TAZ信号通路缓解糖尿病肾病大鼠肾纤维化
- Author:
Xiaodong ZHU
1
;
Xiaoyu WANG
;
Chuan JIANG
;
Chengmei YANG
;
Shumei SHI
Author Information
1. 解放军第九六〇医院肾内科,山东 济南 250031
- Keywords:
asiaticoside;
diabetic nephropathy(DN);
renal fibrosis;
Hippo-YAP/TAZ signaling pathway;
rats
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2025;42(6):1472-1477
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of asiaticoside on renal fibrosis in diabetic nephropathy(DN)rats by regulating the Hippo-yes-associated protein(YAP)/transcriptional co-activator with PDZ-binding motif(TAZ)signaling pathway.Methods A DN rat model was established by feeding a high-sugar and high-fat diet combined with intraperitoneal injection of streptozotocin(STZ).The successfully modeled rats were randomly divided into model group,low-and high-dose asiaticoside groups,and high-dose asiaticoside+XMU-MP-1(Hippo-YAP/TAZ pathway activator)group,with a normal group set as control.After group intervention,blood urea nitrogen(BUN),serum creatinine(SCr),fasting blood glucose(FBG),and 24-hour urinary protein levels were measured.Hematoxylin-eosin(HE)staining was used to observe renal histopathological changes,Masson staining was used to assess renal fibrosis,and Western Blot was used to detect the protein expressions of YAP and TAZ in renal tissue.Results Compared with the normal group,the model group showed disordered renal tissue structure,enlarged and irregular cystic cavities,enlarged renal tubules with thickened basement membranes,inflammatory cell infiltration,and significant collagen deposition in the glomeruli,renal tubules,and vascular walls.The levels of FBG,BUN,SCr,24-hour urinary protein,and the protein expressions of YAP and TAZ in renal tissue were significantly increased(P＜0.05).Compared with the model group,the low-and high-dose asiaticoside groups showed significant improvement in renal tissue damage and fibrosis,with reduced levels of FBG,BUN,SCr,24-hour urinary protein,and decreased protein expressions of YAP and TAZ in renal tissue(P＜0.05).Compared with the high-dose asiaticoside group,the high-dose asiaticoside+XMU-MP-1 group showed a reversal in all the above indicators.Conclusion Asiaticoside may alleviate renal fibrosis and improve renal injury in DN rats by inhibiting the Hippo-YAP/TAZ pathway.
