Effects of Baicalin on Angiogenesis,Inflammatory Response,and Oxidative Stress in Diabetic Foot Ulcer Rats via Modulation of the HIF-1α/VEGF Signaling Pathway
10.13359/j.cnki.gzxbtcm.2025.06.024
- VernacularTitle:黄芩苷调节HIF-1α/VEGF信号通路对糖尿病足溃疡大鼠新生血管生成、炎症反应和氧化应激的影响
- Author:
Guangli LI
1
;
Ping CAO
Author Information
1. 武汉市第三医院内分泌科,湖北 武汉 430060
- Keywords:
baicalin;
diabetic foot ulcer(DFU);
HIF-1α/VEGF pathway;
angiogenesis;
inflammation;
rats
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2025;42(6):1464-1471
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the new therapeutic effects and mechanisms of baicalin on diabetic foot ulcer(DFU)rats.Methods SD rats were randomly divided into a control group,a model group,a low-dose baicalin group,a high-dose baicalin group,and a high-dose baicalin+YC-1[hypoxia-inducible factor 1α(HIF-1α)inhibitor]group.Except for the normal group,the rats in all other groups were constructed to DFU model.After successful modeling,the medication was performed in each group.After treatment,fasting blood glucose level was measured,and the wound healing rate was calculated.Enzyme-linked immunosorbent assay(ELISA)was used to detect the levels of interleukin(IL)-10,tumor necrosis factor(TNF)-α,and IL-6 in wound granulation tissue.The hydroxylamine method was used to measure superoxide dismutase(SOD)activity,the ammonium molybdate method was used to measure catalase(CAT)activity,and the thiobarbituric acid method was used to measure malondialdehyde(MDA)content in wound granulation tissue.Hematoxylin-eosin(HE)staining was used to observe the morphology of wound granulation tissue.Immunohistochemistry was used to detect the positive expression of CD31 protein in wound granulation tissue.Western Blot was used to measure the protein expression levels of advanced glycation end products(AGEs),receptor for advanced glycation end products(RAGE),matrix metalloproteinase 2(MMP-2),HIF-1α,vascular endothelial growth factor(VEGF),and vascular endothelial growth factor receptor 2(VEGFR-2)in wound granulation tissue.Results Compared with the control group,the model group showed reduced angiogenesis and obvious inflanmatory cell infiltration in wound granulation tissue,increased fasting blood glucose level,levels of TNF-α and IL-6,MDA content,and protein expressions of AGEs,RAGE,and MMP-2 in wound tissue,as well as decreased wound healing rate,CD31-positive expression rate,IL-10 level,SOD and CAT activities,and protein expressions of HIF-1α,VEGF,and VEGFR-2,with statistically significant differences(P＜0.05).Compared with the model group,the low-and high-dose baicalin groups showed significantly improved wound granulation tissue morphology,reduced fasting blood glucose level,levels of TNF-α and IL-6,MDA content,and protein expressions of AGEs,RAGE,and MMP-2 in wound tissue,as well as increased wound healing rate,CD31-positive expression rate,IL-10 level,SOD and CAT activities,and protein expressions of HIF-1α,VEGF,and VEGFR-2,with statistically significant differences(P＜0.05).YC-1 partially reversed the improvement effects of baicalin on angiogenesis and inflammation in DFU rats(P＜0.05).Conclusion Baicalin can reduce inflammation and oxidative stress,promote angiogenesis,and accelerate wound healing in DFU rats by activating the HIF-1α/VEGF signaling pathway.
