Baicalin Alleviates Oxidative Stress Injury in Gestational Diabetes Mellitus Rats via Regulating Nrf2 and HO-1
10.13359/j.cnki.gzxbtcm.2025.05.025
- VernacularTitle:黄芩苷调控Nrf2、HO-1缓解妊娠期糖尿病大鼠氧化应激损伤
- Author:
Le YONG
1
;
An WANG
;
Lin CHEN
;
Yuhuan WANG
Author Information
1. 安康市中心医院产科,陕西 安康 725000
- Keywords:
baicalin;
gestational diabetes mellitus;
oxidative stress;
Nrf2/HO-1 signaling pathway;
rats
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2025;42(5):1212-1220
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the therapeutic effect and mechanism of baicalin on gestational diabetes mellitus(GDM)rats.Methods A GDM rat model was induced by a high-fat high-glucose diet combined with Streptozotocin(STZ)Injection.Forty modeled successfully rats were randomly divided into model group,Metformin group,low-and high-dose baicalin group and high-dose baicalin+ML385[nuclear factor erythroid 2-related factor 2(Nrf2)inhibitor]group,with 8 rats in each group,in addition,the normal group(8 healthy rats)was set up.After group intervention,fasting blood glucose(FBG),fasting insulin(FINS),and homeostasis model assessment of insulin resistance(HOMA-IR)levels were measured.Serum levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST),and alkaline phosphatase(ALP)were detected.The levels of superoxide dismutase(SOD),malondialdehyde(MDA),glutathione peroxidase(GSH-Px),and catalase(CAT)in liver tissue were measured.Enzyme-linked immunosorbent assay(ELISA)was used to detect serum inflammatory factors,including tumor necrosis factor α(TNF-α),interleukin 1β(IL-1β),and interleukin 6(IL-6).Hematoxylin-eosin(HE)staining was used to observe liver histopathological changes.Western Blot was used to detect the protein expression levels of Nrf2 and heme oxygenase-1(HO-1)in rat liver tissue.Results Compared with the normal group,the model group showed significantly increased levels of FBG,FINS,HOMA-IR,ALT,AST,ALP,TNF-α,IL-1β,IL-6 and MDA(P＜0.05),while the levels of SOD,CAT,GSH-Px,and the expression levels of Nrf2 and HO-1 were significantly decreased(P＜0.05).HE staining of liver tissue revealed disordered cell arrangement and extensive cell degeneration and necrosis.Compared with the model group,the Metformin group,high-dose baicalin group,and high-dose baicalin+ML385 group showed significantly decreased levels of FBG,FINS,HOMA-IR,ALT,AST,ALP,TNF-α,IL-1β,IL-6 and MDA(P＜0.05),while the levels of SOD,CAT,GSH-Px,and the expression levels of Nrf2 and HO-1 were significantly increased(P＜0.05).The structure of liver sinusoids improved,and necrotic cells were reduced.Compared with the low-dose baicalin group,the high-dose baicalin group showed significantly decreased levels of FBG,FINS,HOMA-IR,ALT,AST,ALP,TNF-α,IL-1β,IL-6 and MDA(P＜0.05),while the levels of SOD,CAT,GSH-Px,and the expression levels of Nrf2 and HO-1 were significantly increased(P＜0.05).The structure of liver sinusoids improved,and necrotic cells were reduced.Compared with the high-dose baicalin group,the high-dose baicalin+ML385 group showed a reversal in the above indicators(P＜0.05).Conclusion Baicalin can alleviate oxidative stress injury in GDM rats by activating Nrf2 and HO-1 expression.
