Exploring the Mechanism of Astragalus-Derived EVs-Like Particles in Diabetic Wound Healing Through Untargeted Metabolomics,Network Pharmacology,and Molecular Docking
10.13359/j.cnki.gzxbtcm.2025.05.024
- VernacularTitle:通过非靶向代谢组学、网络药理学及分子对接探究黄芪来源EVs样颗粒在糖尿病伤口愈合中的作用机制
- Author:
Yanling LI
1
;
Donghao CAI
;
Jiawen SHEN
;
Kewei ZHAO
Author Information
1. 广州中医药大学第三临床学院,广东 广州 510006;中山大学孙逸仙纪念医院输血科,广东 广州 510120
- Keywords:
Astragali Radix-derived EVs-like particles;
diabetes mellitus;
wound healing;
untargeted metabolomics;
network pharmacology;
molecular docking
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2025;42(5):1203-1212
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role and potential mechanism of Astragali Radix-derived extracelluar vesicles(EVs)-like particles(AR-EVLP)in diabetic wound healing,providing a novel therapeutic drug mode and theoretical basis for traditional Chinese medicine in treating diabetic skin ulcers.Methods AR-EVLP was extracted using ultracentrifugation.Untargeted metabolomics was used to analyze the potential active components of AR-EVLP,and the main active substances in AR-EVLP were identified by comparison with the Herb database.The targets of the active components were obtained through the Swiss Target Prediction database and the TCMSP database.Targets related to diabetic wound healing were obtained from the GeneCards database.Key targets were identified by intersecting drug targets and disease targets.GO functional annotation and KEGG pathway enrichment analysis were performed using the DAVID database.Based on the pathway enrichment results,a"drug components-targets-pathways"diagram was constructed to identify core targets.Molecular docking and visualization were performed using Autodock and PyMOL software.Results Formononetin was identified as the main active component in AR-EVLP,with 66 key targets related to diabetes and wound healing,including 15 key node proteins such as SRC,CASP3 and JUN.GO functional enrichment analysis suggested that formononetin can regulate biological processes such as protein phosphorylation and gene expression.KEGG pathway enrichment analysis indicated potential involvement in multiple signaling pathways,including VEGF and TNF.Seven targets,including PIK3CA,JUN and MAPK14,were identified as core targets for formononetin.Molecular docking showed that formononetin had the strongest binding affinity with MAPK14.Conclusion AR-EVLP may be a potential effective drug mode for the treatment of diabetic wound healing.
