Study on the Distribution of Traditional Chinese Medicine Syndrome Types in Patients with Lumbar Disc Herniation and Its Correlation with Serum HIF-1α,NLRP3 and IL-1β Levels
10.13359/j.cnki.gzxbtcm.2025.05.004
- VernacularTitle:腰椎间盘突出症患者中医证型分布及其与血清HIF-1α、NLRP3、IL-1β水平的关系研究
- Author:
Juan CHEN
1
;
Guoqi LEI
Author Information
1. 武汉市汉口医院,湖北 武汉 432001
- Keywords:
lumbar disc herniation;
traditional Chinese medicine(TCM)syndrome types;
hypoxia-inducible factor 1α;
NOD-like receptor thermal protein domain associated protein 3;
interleukin-1β;
prognosis;
correlation;
biological indicators
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2025;42(5):1066-1072
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the distribution of traditional Chinese medicine(TCM)syndrome types in patients with lumbar disc herniation(LDH)and its correlation with serum levels of hypoxia-inducible factor 1α(HIF-1α),NOD-like receptor thermal protein domain associated protein 3(NLRP3)and interleukin 1β(IL-1β)of the patients.Methods Data collection was conducted in 160 patients(LDH group)admitted to the LDH outpatient and inpatient department of Wuhan Hankou Hospital from October 2022 to June 2024 and in 20 healthy volunteers(healthy control group)who underwent physical examination in the hospital during the same period.The levels of serum HIF-1α,NLRP3 and IL-1β were compared between the two groups,and then the correlation of serum HIF-1α,NLRP3 and IL-1β levels with Visual Analogue Scale(VAS)score of pain,Japanese Orthopedic Association(JOA)score and Oswestry Dysfunction Index(ODI)score in LDH patients was explored.The distribution of TCM syndrome types in LDH patients was statistically analyzed,and then the correlation between TCM syndromes and serum HIF-1α,NLRP3 and IL-1β levels in LDH patients was investigated.Furthermore,the correlation between serum HIF-1α,NLRP3,IL-1β levels and prognosis of LDH was analyzed.Results(1)Compared with the healthy control group,the serum levels of HIF-1α,NLRP3 and IL-1β in the LDH group were significantly increased,and the differences were statistically significant(P＜0.01).(2)Spearman correlation analysis showed that VAS and ODI scores were positively correlated with serum HIF-1α,NLRP3 and IL-1β levels,and JOA score was negatively correlated with serum HIF-1α,NLRP3 and IL-1β levels,the differences being statistically significant(P＜0.01).(3)The percentage of TCM syndrome types in 160 patients with LDH in descending order was as follows:blood stasis syndrome(65 cases,40.63%)＞kidney deficiency syndrome(45 cases,28.13%)＞cold-damp syndrome(30 cases,18.75%)＞damp-heat syndrome(20 cases,12.50%).(4)There were statistically significant differences in serum levels of HIF-1α,NLRP3 and IL-1β among LDH patients with different TCM syndromes(P＜0.01):the serum levels in patients with cold-damp syndrome were higher than those in patients with kidney deficiency syndrome(P＜0.05),serum levels in patients with damp-heat syndrome were higher than those in patients with cold-damp syndrome(P＜0.05),and serum levels in patients with blood stasis syndrome were higher than those in patients with damp-heat syndrome(P＜0.05).(5)Multivariate logistic regression analysis showed that serum HIF-1α,NLRP3 and IL-1βwere risk factors for the prognosis of LDH patients,and they were negatively correlated with the prognosis of LDH(P＜0.05 or P＜0.01).Conclusion Blood stasis syndrome is the predominated syndrome type in LDH patients,and then comes kidney deficiency syndrome.The levels of serum HIF-1α,NLRP3 and IL-1β vary in LDH patients with different TCM syndrome types,and are expected to be the potential biological indicators for TCM syndrome differentiation of LDH.Serum HIF-1α,NLRP3 and IL-1β are the risk factors for the prognosis of LDH.
