Non-targeted Lipid Metabolomics of Tongue Coating in Patients with Ischemic Stroke
10.13359/j.cnki.gzxbtcm.2025.05.003
- VernacularTitle:缺血性中风患者舌苔非靶向脂质代谢组学研究
- Author:
Cuicui LI
1
;
Donghao CAI
;
Jingwen XU
;
Hui LI
Author Information
1. 广东省第二中医院,广东 广州 510095
- Keywords:
ischemic stroke;
tongue coating;
lipid metabolites;
non-targeted lipid metabolomics;
ultra high-performance liquid chromatography-mass spectrometry technology;
UHPLC-MS;
potential biomarkers
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2025;42(5):1060-1065
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the differences of lipid metabolites in tongue coating between ischemic stroke patients and healthy people,thus to screen out the potential biomarkers for tongue coating of ischemic stroke patients.Methods Twenty patients confirmed as ischemic stroke were selected as the observation group,and another 20 controls receiving healthy medical checkup at the same period were selected as the control group.All of them were aged between 40 and 80 years.Using ultra-high performance liquid chromatography-mass spectrometry(UHPLC-MS)technology,and by combined with the statistical methods of principal component analysis(PCA)and partial least squares discriminant analysis(PLS-DA),non-targeted lipid metabolomics study was performed on tongue coating samples of the observation group and the control group.According to the criteria that the VIP value in the orthogonal signal correction-based orthogonal partial least squares discriminant analysis(OPLS-DA)model was greater than 1 and the P value of the Wilcoxon rank test was less than 0.05,lipid metabolites with significant differences were screened and determined.Results Differential lipid metabolites exist in ischemic stroke patients(observation group)and healthy people(control group),and a total of 14 lipid metabolites were identified,and they were free fatty acids,ceramide,sphingomyelin,cardiolipin,lecithin,etc.Of the 14 lipid metabolites,4 were down-regulated and 10 were up-regulated,and the differences were statistically significant(P＜0.05).Conclusion There exist differential lipid metabolites in tongue coating of patients with ischemic stroke and the healthy controls,and these different lipid metabolites are expected to become potential biomarkers for ischemic stroke.
