Research and Application of Quality Evaluation of Ganoderma lucidum Based on the Combination of DNA Molecular Identification and Physicochemical Analysis
10.13359/j.cnki.gzxbtcm.2025.04.027
- VernacularTitle:DNA分子鉴定和理化分析相结合对灵芝质量评价的研究与应用
- Author:
Qingying ZHANG
1
;
Yanyan DONG
Author Information
1. 聊城职业技术学院,山东聊城 252000
- Keywords:
Ganoderma;
DNA molecular identification;
physicochemical analysis;
quality evaluation
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2025;42(4):988-991
- CountryChina
- Language:Chinese
-
Abstract:
Objective The quality evaluation of 20 batches of Ganoderma was carried out by the combination of DNA identification and physicochemical analysis.Methods The 20 batches of Ganoderma were identified by DNA extraction,polymerase chain reaction(PCR)amplification,purification,sequencing,and inputting the Chinese medicines into DNA barcode identification system.Then the content determination of marker components was carried out.Results Ganoderma of No.1,2,3,4,5,6,9,10,11,12,13,14,15,16,18 and 20 belonged to Ganoderma lucidum(Leyss.ex Fr.)Karst.in the Pharmacopoeia,while No.7,8,17 and 19 did not belong to Ganoderma lucidum(Leyss.ex Fr.)Karst.in the Pharmacopoeia.Content determination was conducted on 16 batches of Ganoderma lucidum(Leyss.ex Fr.)Karst.,in which 15 batches were up to the standards of Pharmacopoeia,the decreasing sequence of contents was as follows:No.2,1,5,10,18,3,4,16,15,11,13,14,6,9,and 12,and No.20 was failed to meet the standards,with a content of 0.38％.Conclusion The combination of DNA molecular identification and physicochemical analysis can be used for rapid quality evaluation of the authenticity and superiority of Ganoderma.
